Culture medium for culturing bacillus subtilis for feedstuffs

A technology of Bacillus subtilis and culture medium, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., to achieve the effect of solving polluted environment, high production cost, and reducing culture cost

Inactive Publication Date: 2012-11-21
龙源海洋生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its disadvantage is that the production cost is high

Method used

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  • Culture medium for culturing bacillus subtilis for feedstuffs
  • Culture medium for culturing bacillus subtilis for feedstuffs
  • Culture medium for culturing bacillus subtilis for feedstuffs

Examples

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example 1

[0023] Accurately weigh 10 grams of glucose, 5 grams of sodium chloride, potassium dihydrogen phosphate 3 and 1.5 grams of magnesium sulfate, put them in a beaker, and then weigh 20 grams of fishmeal wastewater concentrate, and then add 800 grams of water to the beaker and stir evenly , Heating, after the above components are completely dissolved, adjust the pH to 7.0 with 5mol / L NaOH, and add water to 1000 ml. Divide the prepared medium into 250 ml Erlenmeyer flasks, each with a volume of 60 ml, seal with a breathable sealing film, and autoclave at 121.3°C for 30 minutes.

example 2

[0025] Accurately weigh 10 grams of glucose, 5 grams of sodium chloride, potassium dihydrogen phosphate 3 and 1.5 grams of magnesium sulfate, put them into a beaker, and then weigh 50 grams of fishmeal wastewater concentrate, and then add 800 grams of water to the beaker and stir evenly , Heating, after the above components are completely dissolved, adjust the pH to 7.0 with 1mol / L NaOH, and add water to 1000 ml. Divide the prepared medium into 250 ml Erlenmeyer flasks, each with a volume of 60 ml, seal with a breathable sealing film, and autoclave at 121.3°C for 30 minutes.

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Abstract

The invention relates to a culture medium for culturing bacillus subtilis for feedstuffs. The culture medium comprises the following components in percentage by weight: 2%-5% of fish meal waste water concentrated solution, 1% of glucose, 0.5% of sodium chloride, 0.3% of monopotassium phosphate, 0.15% of magnesium sulfate and the balance of water. According to the culture medium for culturing the bacillus subtilis for the feedstuffs disclosed by the invention, the fish meal waste water concentrated solution is used as a new raw material for producing the bacillus subtilis BL-8, and the culturing cost of the bacillus subtilis can be greatly reduced; the number of zymocytes of the bacillus is improved, and spore production rate of the bacillus subtilis is improved; and a new way is developed for the use of the fish meal waste water, and the problems that the environment is polluted by the fish meal waste water which is a by-product in the fish meal production and the fish meal waste water is difficult to treat are solved.

Description

Technical field: [0001] The invention relates to a bacillus culture medium for feed. Background technique: [0002] Bacillus subtilis LB-8 is quoted from the China Microorganism Collection Center, and the preservation number is: CGMCC No.1.210. It is a bacillus that can be used for feed. It has a strong ability to synthesize α-amylase. It is used in animal husbandry, poultry, aquatic products, Special animal and pet breeding can prevent animal indigestion and improve the utilization rate of feed; it can also prevent animal diarrhea, diarrhea and other gastrointestinal diseases; Bacillus subtilis LB-8 exists in the form of endogenous spores and is a robust Living cells in a dormant state have strong stress resistance and long storage time. They are relatively stable no matter in pellet feed or powder feed, and can smoothly enter the intestinal tract of animals to survive and proliferate in large quantities. The feed industry has been popularized and applied for many years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 刘君冯源杨建国
Owner 龙源海洋生物股份有限公司
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