Method for immobilizing heparanase I
A technology of heparinase and chitosan microspheres, which is applied in the direction of immobilization on/in the organic carrier, can solve the problems of low immobilization efficiency and high cost, and achieve high stability, high immobilization efficiency, high The effect of reusability
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[0038] The heparinase used is isolated and purified from the fermented and cultured cells of Flavobacterium heparinus. For the enzyme preparation process, refer to the example in "A Preparation Method for Flavobacterium Heparinus Heparinase I" of Invention Patent Application No. 200910039360.1.
[0039] a, getting 1g of commercially available chitosan powder with a purity of 99% is dissolved in 50ml of 2% acetic acid to obtain an acidic chitosan sol, and 0.6g of commercially available chemically pure Span 80 (Sinopharm Chemical Reagent Co., Ltd. , chemically pure) was added into 100ml of liquid paraffin, stirred and mixed at a speed of 800rpm, the acidic chitosan sol was added dropwise into the liquid paraffin, after emulsification for 1h, 5ml of glutaraldehyde with a mass fraction of 25% was added as a crosslinking agent, The crosslinking temperature is 40°C, add 100ml equal volume of 2.5M NaOH and absolute ethanol mixture after 1h, let it stand for 10min after stirring, disca...
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