Method for preparing magnetic covalent immobilized enzyme carriers

Abstract

The invention discloses a method for preparing magnetic covalent immobilized enzyme carriers, which relates to the field of biological chemistry and comprises the following steps that: firstly, oleic acid covered Fe3O4 with a magnetic structure is prepared, and then, Fe3O4 carries out polymerization reaction with olefine acid ester and subsequently and sequentially carries out reaction with hydrazine hydrate and nitrous acid to obtain activated immobilized enzyme carriers in nitrine magnetic nanometer structures. The method of the invention has the advantages that the process is simple, the operation cost is low, the enzyme carrying quantity is high, the stability is good, the reaction is easy to control and operate, the enzyme carrying capacity is as high as 250mg / g, and the original enzyme performance of the immobilized enzyme can almost be maintained at 100 percent.

Description

technical field

[0001] The invention relates to the field of biochemistry, in particular to a method for preparing a magnetic covalently immobilized enzyme carrier. Background technique

[0002] Immobilized enzyme refers to an enzyme preparation that is not easy to lose with water, that is, its movement is restricted, and can play a catalytic role after being treated by physical or chemical methods. Compared with free enzymes, the advantages of immobilized enzymes are: it can be used multiple times, and the stability of the enzyme is improved; after the reaction, the immobilized enzyme is easy to separate from the substrate and product; the reaction conditions are easy to control, which is conducive to automated production.

[0003] Magnetic nanostructured Fe 3 o 4 It has attracted extensive attention because of its biocompatibility, small size effect, large surface area, and special magnetic properties. Fe 3 o 4 The literature on covalent immobilization of enzymes as c...