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Method for preparing immobilized cell carrier from tamarind seed gum and sodium alginate complex gel

A technology of immobilizing cells and sodium alginate is applied in the material field of immobilized cell carriers, which can solve the problems of reducing the efficiency of immobilization, the loss of microbial activity, and the loss of microorganisms, so as to reduce the loss of activity, reduce the loss of microorganisms, and reduce the loss of microorganisms. The effect of fast forming

Inactive Publication Date: 2011-02-16
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

1. Due to the performance of carrageenan itself, the curing temperature is 35°C. During the operation of curing cells, the loss of microbial activity is still relatively large; 2. Although the mechanical strength of the carrier material reaches 1.70-1.90kg / g, However, after repeated use for many times, there will be damage, resulting in the loss of microorganisms, thereby reducing the efficiency of immobilization; 3. According to DSC thermal analysis, the decomposition temperature of the immobilized cell carrier is 243.8°C, and the heat resistance performance is still to be determined. Further improve

Method used

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  • Method for preparing immobilized cell carrier from tamarind seed gum and sodium alginate complex gel
  • Method for preparing immobilized cell carrier from tamarind seed gum and sodium alginate complex gel
  • Method for preparing immobilized cell carrier from tamarind seed gum and sodium alginate complex gel

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Experimental program
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Effect test

Embodiment 1

[0025] 1) Under the condition that the temperature is 85°C and the rotating speed is 500 rpm, a composite aqueous solution of tamarind gum and sodium alginate with a concentration of 2.4% by weight is prepared, and the mass ratio of tamarind gum and sodium alginate is 5: 1. Stir at constant temperature for 25 minutes to obtain a compound solution of tamarind gum and sodium alginate, then cool to room temperature and set aside;

[0026] 2) At a temperature of 3°C and a speed of 120 rpm, use a syringe with a No. 7 needle to drop the compound solution obtained in step 1) into a calcium chloride solution with a concentration of 2.5% by weight, and compound The volume ratio of the solution and the calcium chloride solution was 1:10, and the constant temperature curing reaction was carried out for 2 hours, and a composite gel of tamarind gum and sodium alginate was initially formed to immobilize the cell carrier.

[0027] 3) At room temperature, remove the calcium chloride solution ...

Embodiment 2

[0030] 1) Under the condition that the temperature is 95°C and the rotating speed is 600 rpm, a composite aqueous solution of tamarind gum and sodium alginate with a concentration of 3.0% by weight is prepared, and the mass ratio of tamarind gum and sodium alginate is 4: 1. Stir at constant temperature for 35 minutes to obtain a composite solution of tamarind gum and sodium alginate, then cool to room temperature and set aside;

[0031] 2) At a temperature of 5°C and a speed of 150 rpm, use a syringe with a No. 7 needle to drop the compound solution obtained in step 1) into a calcium chloride solution with a concentration of 3.5% by weight, and compound The volume ratio of the solution and the calcium chloride solution is 1.5:10, and the curing reaction is carried out at a constant temperature for 2 hours, and a composite gel of tamarind gum and sodium alginate is initially formed to immobilize the cell carrier;

[0032] 3) At room temperature, remove the calcium chloride solu...

Embodiment 3

[0035] 1) Under the condition that the temperature is 90°C and the rotating speed is 550 rpm, a composite aqueous solution of tamarind gum and sodium alginate with a concentration of 2.7% by weight is prepared, and the mass ratio of tamarind gum and sodium alginate is 4.5: 1. Stir at constant temperature for 30 minutes to obtain a compound solution of tamarind gum and sodium alginate, then cool to room temperature and set aside;

[0036] 2) at a temperature of 4°C and at a speed of 135 rev / min, drop the composite solution of bentonite into the calcium chloride solution of 3.0% by weight with a syringe with a No. 7 needle, the composite solution and chloride The volume ratio of the calcium solution is 1.25:10, and the constant temperature curing reaction is carried out for 1.5 hours, and the composite gel of tamarind gum and sodium alginate is initially formed to immobilize the cell carrier;

[0037] 3) At room temperature, remove the calcium chloride solution by filtration, wa...

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Abstract

The invention relates to the field of polymer materials. The gel raw materials of an immobilized cell carrier which is prepared from bentonite and biological polysaccharide complex gel and disclosed in the patent with the application number of 200710151906.3 comprise carrageenan and guar gum complex natural plant polysaccharide. Compared with the prior art, various properties of the immobilized cell carrier are enhanced, but the curing temperature is higher, and the thermal decomposition temperature as well as the mechanical strength are lower, thereby causing more loss of microbial activities and larger damage rate of a cell carrier ball. In order to further improve the properties, the method adopts the following steps: preparing a water solution with the consistency of 2.4-3.0 percent from tamarind seed gum and sodium alginate complex biological polysaccharide; curing the water solution after cooling in a calcium chloride solution with the consistency of 2.5-3.5 percent and a sodiumpyroborate solution with the consistency of 2.5-3.5 percent for the second time; and finally, keeping the cured solution in physiological saline. The prepared immobilized cell carrier has the advantages of high mechanical strength, thermal decomposition temperature and microbial curing rate as well as low damage rate and cost.

Description

technical field [0001] The invention relates to the field of functional polymer materials, especially the material of immobilized cell carriers. Background technique [0002] Immobilized cell carrier is a new type of functional polymer material developed in the past thirty years. Immobilized cell carrier balls can be used to embed and covalently bond biologically active substances such as plants, animals, and microbial cells, so that the biologically active substances have good operability and production performance, and can be used in various fields. [0003] Scholars at home and abroad attach great importance to the study of immobilized cell carriers. We have studied a variety of immobilized carriers, and we applied for "Bentonite, biopolysaccharide composite gel immobilized cell carrier and its preparation method" on September 20, 2007. The application number is 200710151906.3. The patent application for this invention has the following characteristics: [0004] 1. Usin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04
Inventor 张继许生军杜兆莉周文芳梁俊玉姚健马军义
Owner NORTHWEST NORMAL UNIVERSITY
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