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Biocompatible metal-organic framework material glutamic acid-zinc and preparation and application thereof

A metal-organic framework and biocompatibility technology, which is applied in the field of biocompatible metal-organic framework material glutamate-zinc and its preparation and application, can solve the problems of slow speed and low solubility, and achieve mild reaction and fast production speed , The effect of high recovery rate of enzyme activity

Active Publication Date: 2017-07-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glutamic acid has low solubility in cold water and organic solvents, and it is a weak acid that is not easily ionized into -COO - , resulting in a Zn 2+ Generate Zn(L-Glu)(H 2 O)]H 2 O is very slow

Method used

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  • Biocompatible metal-organic framework material glutamic acid-zinc and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) 15mL containing 1.8mmol disodium glutamate aqueous solution was added dropwise to 45mL containing 3mmol Zn(NO 3 ) 2 in methanol (disodium glutamate and Zn(NO 3 ) 2 The molar ratio of 3:5; the volume ratio of disodium glutamate aqueous solution to methanol is 1:3, containing Zn(NO 3 ) 2 Zn(NO 3 ) 2 concentration is 0.05mol / L), immediately obtain white precipitate, centrifuge and freeze-dry after washing, obtain biocompatible metal-organic framework material glutamic acid-zinc carrier material (Zn(L-Glu)(H 2 O)]H 2 O)( figure 1 );

[0043] (2) Aspergillus niger lipase is dispersed in the phosphate buffer solution that pH is 8, obtains enzyme solution (20mg / mL);

[0044](3) At 25°C, add 2mg of EDC·HCl to 1mL enzyme solution and stir at 150rpm for 1h to obtain mixed solution A;

[0045] (4) Add 2.4 mg of NHS to the mixed solution A obtained in step (3) at 25° C. and continue stirring for 1 hour to obtain mixed solution B;

[0046] (5) Take 44 mg of the biocom...

Embodiment 2

[0048] (1) 15mL aqueous solution containing 1.5mmol disodium glutamate was added dropwise to 45mL containing 3mmol Zn(NO 3 ) 2 in methanol (disodium glutamate and Zn(NO 3 ) 2 The molar ratio of 1:2; the volume ratio of disodium glutamate aqueous solution to methanol is 1:3, containing Zn(NO 3 ) 2 Zn(NO 3 ) 2 The concentration is 0.05mol / L), obtain white precipitate immediately, centrifuge and freeze-dry after washing, obtain biocompatible metal-organic framework material glutamic acid-zinc carrier material (Zn(L-Glu)(H 2 O)]H 2 O);

[0049] (2) Aspergillus niger lipase is dispersed in the phosphate buffer solution that pH is 8, obtains enzyme solution (20mg / mL);

[0050] (3) At 25°C, add 2mg of EDC·HCl to 1mL enzyme solution and stir at 150rpm for 1h to obtain mixed solution A;

[0051] (4) Add 2.4 mg of NHS to the mixed solution A obtained in step (3) at 25°C and continue stirring at 150 rpm for 1 hour to obtain mixed solution B;

[0052] (5) Take 44 mg of the bioco...

Embodiment 3

[0054] (1) 15mL aqueous solution containing 1.0mmol disodium glutamate was added dropwise to 45mL containing 3mmol Zn(NO 3 ) 2 in methanol (disodium glutamate and Zn(NO 3 ) 2 The molar ratio of 1:3; the volume ratio of disodium glutamate aqueous solution to methanol is 1:3, containing Zn(NO 3 ) 2 Zn(NO 3 ) 2 The concentration is 0.05mol / L), obtain white precipitate immediately, centrifuge and freeze-dry after washing, obtain biocompatible metal-organic framework material glutamic acid-zinc carrier material (Zn(L-Glu)(H 2 O)]H 2 O);

[0055] (2) Aspergillus niger lipase is dispersed in the phosphate buffer solution that pH is 8, obtains enzyme solution (20mg / mL);

[0056] (3) At 25°C, add 2mg of EDC·HCl to 1mL enzyme solution and stir at 150rpm for 1h to obtain mixed solution A;

[0057] (4) Add 2.4 mg of NHS to the mixed solution A obtained in step (3) at 25°C and continue stirring at 150 rpm for 1 hour to obtain mixed solution B;

[0058] (5) Take 44 mg of the bioco...

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Abstract

The invention belongs to the technical field of novel materials and particularly relates to a biocompatible metal-organic framework material glutamic acid-zinc and preparation and an application thereof. The biocompatible metal-organic framework material glutamic acid-zinc is obtained through dropwise adding a disodium glutamate water solution into a methanol solution of Zn(NO3)2. The method has the characteristics of being high in glutamic acid-zinc crystal growth speed and mild in preparation condition, and the prepared product is good in crystal form, can be applied to a series of enzymes containing abundant amino, such as lipase, protease and epoxide hydrolase, and has the potential as a protein drug carrier. The product is added to an aspergillus niger lipase phosphate buffer solution modified by EDC or EDC.HC and NHS for shock immobilization to obtain the immobilized aspergillus niger lipase. The method is high in immobilization efficiency, high in enzyme recovery rate, simple in operation and mild in condition.

Description

[0001] The patent application of the present invention is a divisional application with the application number "201610113094.2", the original application date is "February 29, 2016", the application number is "201610113094.2", and the invention name is "A biocompatible metal organic framework Material glutamate-zinc and its preparation and application". technical field [0002] The invention belongs to the technical field of new materials, and in particular relates to a biocompatible metal organic framework material glutamic acid-zinc and its preparation and application. Background technique [0003] Enzyme immobilization on metal-organic frameworks (MOFs) is a research hotspot at home and abroad because of its unique and diverse topology, porosity, and physical properties. Metal-organic framework materials have many properties such as porosity, large specific surface area, and multiple metal sites, and have good application prospects in the fields of gas storage, molecular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C07C227/18C07C229/76
CPCC07C227/18C07C229/76C12N9/20C12N11/02C12Y301/01003
Inventor 娄文勇夏高辉曹诗林宗敏华
Owner SOUTH CHINA UNIV OF TECH
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