Anti-early diabetic nephropathy drug screening method based on podocyte adhesion

A technology of podocytes and drugs, applied in the field of real-time dynamic high-throughput screening of anti-early diabetic nephropathy drugs based on podocyte adhesion, can solve the problems of insufficient and difficult functional research, and achieve stability and reliability , the process is simple, and the effect of accelerating the discovery process

Active Publication Date: 2016-01-20
SHANGHAI SIXTH PEOPLES HOSPITAL
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Problems solved by technology

[0005] Especially for traditional Chinese medicine, although it has the advantages of less toxic and side effects, there are many kinds of traditional Chinese medicine, and its function research is not enough. Therefore, it is not easy to choose a drug that can resist early diabetic nephropathy among many Chinese herbal medicines. A method that can quickly screen anti-diabetic nephropathy drugs is not only critical for the treatment of diabetic nephropathy, but also has great significance for the development and utilization of traditional Chinese medicine

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  • Anti-early diabetic nephropathy drug screening method based on podocyte adhesion
  • Anti-early diabetic nephropathy drug screening method based on podocyte adhesion
  • Anti-early diabetic nephropathy drug screening method based on podocyte adhesion

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Embodiment Construction

[0026] Disclosed in the present invention is a screening method for anti-early diabetic nephropathy drugs. By adopting xCELLigence system technology to establish a real-time dynamic high-throughput drug screening method based on podocyte adhesion, the drug that increases podocyte adhesion is preliminarily screened at the cellular level. Compounds, dynamic detection of podocyte adhesion, real-time observation of cell growth, drug effect initiation time point and maximum action time point; and quantitative analysis and sorting of single cells at the molecular level using flow cytometry, high-speed analysis of tens of thousands individual cells and simultaneously monitor multiple parameters in the cell.

[0027] The xCELLigence system uses a cell analyzer jointly developed by Roche and ACEABiosciences.

[0028] Flow cytometer is a device for automatic analysis and sorting of cells. It can quickly measure, store, and display a series of important biophysical and biochemical charac...

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Abstract

The invention provides a sertoli cell adhesion-based screening method for anti-early-stage diabetic nephropathy drugs. The method increases the sertoli cell adhesion compounds via a preliminary screening at a cellular level with an xCELLigence system, and further combines a flow cytomeytry to perform quantitative analysis and sorting on single cells at the molecular level, so that screening accuracy further ensured. Real-time dynamic high throughput screening method for the anti-early-stage diabetic nephropathy drugs has the characteristics of no damage, real-time dynamic detection, rich information, simple process and good repeatability.

Description

technical field [0001] The invention relates to a method for screening drugs, in particular to a method for real-time dynamic high-throughput screening of anti-early diabetic nephropathy drugs based on podocyte adhesion. Background technique [0002] The application of drugs is a very complicated research, and improving the accurate application of drugs is of great significance in medical research. Drug screening refers to the screening of drugs that are put into application and the evaluation of the value of drugs. The more samples screened, the greater the possibility of finding highly targeted and suitable drugs. Therefore, high-throughput drug screening technology has become the mainstay of drug screening important platform. [0003] High-throughput screening technology (High-throughput Screening, HTS) is a high-tech drug search formed in the late 1980s. A technical system more suitable for drug screening. High-throughput screening technology is based on experimental ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14C12Q1/06
Inventor 汪年松桂定坤王筱霞王锋范瑛
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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