Kit for detecting H3N2 avian influenza virus
An avian influenza virus, H3N2 technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve the problems of lack of simple, fast, easy-to-use detection methods, etc., and achieve high sensitivity, improved sensitivity, and simple operation. Effect
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[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H3N2 type avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H3N2 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system that amplifies H3N2 avian influenza virus RNA in a sample to be detected after a reverse transcription process.
[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.
[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol...
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