Kit for detecting H3N2 avian influenza virus

An avian influenza virus, H3N2 technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve the problems of lack of simple, fast, easy-to-use detection methods, etc., and achieve high sensitivity, improved sensitivity, and simple operation. Effect

Inactive Publication Date: 2013-01-02
天津朝海科技有限公司
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AI Technical Summary

Problems solved by technology

[0006] In January 2006, H3N2 avian influenza virus appeared in the United States. Currently, there is no simple, rapid, easy-to-use and low-cost detection method for H3N2 avian influenza virus

Method used

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  • Kit for detecting H3N2 avian influenza virus

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Embodiment Construction

[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H3N2 type avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H3N2 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system that amplifies H3N2 avian influenza virus RNA in a sample to be detected after a reverse transcription process.

[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol...

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Abstract

The invention relates to a kit for detecting DNA (deoxyribose nucleic acid) of a product of H3N2 avian influenza virus through gold nanoparticles after the product is amplified via a loop-mediated isothermal nucleic acid amplification technology, belonging to the technical field of biological detection. According to the kit, the loop-mediated isothermal nucleic acid amplification technology and a bio nanotechnology are combined, therefore, the sensitivity in detection of biomolecule is improved, and a method which can be simply and fast operated along with accuracy without professional instrument and equipment is provided; and the kit can be widely applied to detecting high-sensitivity H3N2 avian influenza virus in the fields of family diagnosis, clinical diagnosis, communicable disease control, environment monitoring, inspection and quarantine and biotechnology and the like. The kit provided by the invention includes two parts which are respectively (1) gold nanoparticles on which molecular probes for distinguishing the special sequence of the H3N2 avian influenza virus are marked, and (2) the loop-mediated isothermal nucleic acid amplification system for amplifying the N2 avian influenza DNA of a sample to be detected or amplifying the H3N2 avian influenza RNA in the sample to be detected after carrying out reverse transcription.

Description

technical field [0001] The invention relates to a kit for detecting H3N2 avian influenza virus DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), ref...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 不公告发明人
Owner 天津朝海科技有限公司
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