Application of Houttuynoid A in antibacterial medicine
An anti-bacterial and drug technology, applied in the direction of anti-bacterial drugs, resistance to vector-borne diseases, pharmaceutical formulations, etc., can solve the problems of digestive tract irritation, strong accumulation of toxicity, etc.
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Embodiment 1
[0012] Embodiment 1: the preparation of compound Houttuynoid A tablet involved in the present invention:
[0013] Get 20 grams of compound Houttuynoid A, add 180 grams of conventional adjuvants for preparing tablets, mix well, and make 1000 tablets on a conventional tablet press.
Embodiment 2
[0014] Embodiment 2: the preparation of compound Houttuynoid A capsule involved in the present invention:
[0015] Get 20 grams of compound Houttuynoid A, add conventional auxiliary materials such as starch 180 grams for preparing capsules, mix well, and pack into capsules to make 1000 tablets.
[0016] The following pharmacodynamic experiments will further illustrate its drug activity.
experiment example
[0017] Experimental example: Antibacterial activity of Houttuynoid A
[0018] The antimicrobial activity test adopts the method of concentration dilution, and each measurement is repeated three times. The test pathogens include Escherichia coli, Pseudomonas fluorescens, Staphylococcus aureus, Proteus, and Cryptococcus neoformans, and the concentration of the bacterial solution is 10 5 individual / mL. The initial concentration of Houttuynoid A was 50.0 μg / mL (5% dimethyl sulfoxide DMSO), and it was serially diluted to 0.098 μg / mL. The same volume of bacterial liquid and test samples were mixed and cultured in a 96-well plate, and the bacterial culture temperatures were respectively 37°C, observe after 24 hours of incubation time, if no colonies are found, it is the lowest antibacterial concentration of the sample, that is, the MIC value. The positive control of this experiment was amikacin sulfate, and the antibacterial results of Houttuynoid A are shown in Table 1.
[0019] T...
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