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Method for quickly detecting concentrations of various electrolytes by ion-selective electrodes
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An ion-selective electrode and ion-selective technology, applied in the field of ion detection, can solve problems such as inability to perform quantitative testing
Active Publication Date: 2013-01-09
深圳市希莱恒医用电子有限公司
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Problems solved by technology
The above tests of magnesium, whether done manually or using biochemical instruments, are all qualitative measurements and cannot be quantitatively tested.
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Embodiment 1
[0010] Example 1 Preparation of calibration solution A and slope solution B.
[0011] According to the test K / Na / Cl / Ca / pH / Li / Mg / CO 2 In order to meet the needs of the electrolyte analyzer with eight parameters, it is necessary to accurately prepare the standard liquid A and the slope liquid B.
[0012] The concentration requirement of each ion in calibration solution A is: K + =4mmol / L, Na + =140mmol / L, Cl - =100mmol / L, Ca 2+ =1.25mmol / L, PH=7.38, Li=1.00mmol / L, Mg 2+ =0.75mmol / L, CO 2 =20mmol / L.
[0013] The concentration requirement of each ion in the slope liquid B liquid is: K + =8mmol / L, Na + =110mmol / L, Cl - =70mmol / L, Ca 2+ =2.5mmol / L, PH=6.84, Li=2.00mmol / L, Mg 2+ =1.5mmol / L, CO 2 =10mmol / L.
[0014] According to the required concentration above, accurately measure each reagent in the list below for preparation.
[0018] In the eight-item electrolyte analyzer of Xilaiheng, use the above-mentioned calibration solution A and slope solution B to perform two-point calibration, and test the slope of each electrode. The slopes are recorded as follows.
[0019] parameter K Na Cl Ca pH Li Mg A standard potential / mv 79.4 82.1 70.3 84.4 80.1 75.3 84.9 B standard potential / mv 94.9 76.2 77.3 92.8 116.1 90.6 93.9 slope 51.7 58.4 46.7 28 60 51 30
[0020] Among them, CO2 is tested with a pressure sensor, and no two-point calibration is required, only one-point calibration is required.
Embodiment 3
[0021] Example 3 Accuracy Measurements.
[0022] The eight-parameter electrolyte analyzer of the present invention was compared with the Johnson & Johnson dry-type biochemical analyzer, and the test results are as follows (unit: mmol / L).
[0023]
[0024]
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Abstract
The invention relates to ion detection, in particular to a method for quickly detecting concentrations of various electrolytes by ion-selective electrodes. The method comprises the steps of: (1) adopting ion-selective electrodes for K / Na / Cl / Ca / pH / Li / Mg, absorbing an A / B liquid first to calibrate, calculating the gradient and other parameters of each electrode and establishing a coordinate curve of each electrode item by the Nernst equation, and then testing an unknown solution sample; and (2) determining the ion concentration of the unknown solution sample according to coordinates established previously, using a high precision pressure sensor to detect concentration of CO2, and calculating the CO2 concentration of the unknown solution by calibrating a solution of a standard concentration of CO2 by means of direct proportion relation of concentration of CO2 solution and generated pressure. The method can measure eight parameters of K / Na / Cl / Ca / pH / Li / Mg / CO2 of the solution at one time without sampling for many times to detect.
Description
technical field [0001] The invention relates to ion detection, in particular to a method for rapidly detecting the concentration of various electrolytes in a solution by using an ion selective electrode. Background technique [0002] Electrolyte analyzers in the prior art can only quantitatively test the five items of K / Na / Cl / Ca / pH in the solution, and generally use biochemical methods for semi-quantitative testing. But the quantitative test of magnesium has not been a good solution. Mg is one of the essential elements of the human body. The content of magnesium in intracellular cations is second only to potassium. Magnesium is widely present in various tissues in the body, participates in many biological processes, and maintains muscle contraction and nerve stress. And can activate many enzymes in the body to promote energy metabolism. The test of conventional magnesium generally adopts colorimetric method, such as serum magnesium (Mg) test box, and its test principle is:...
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