Method for preparing super cytokine induced killer (CIK) cells
A cell-killing and super-technology, applied in the medical field, can solve the problems of severe toxic and side effects, and achieve the effects of reducing side effects, avoiding toxic and side effects, and avoiding immune escape
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[0023] For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed.
[0024] 1. Preparation of CIK cells
[0025] 1. The preparation of super CIK cells of the present invention, such as figure 1 Shown:
[0026] (1) Collection and preparation of peripheral blood mononuclear cells (PBMC)
[0027] Use a blood cell separator to collect anticoagulant blood from tumor patients under sterile conditions, add Ficoll-Paque Plus and blood with a specific gravity of 1.077 to a centrifuge tube at a ratio of 1:2, centrifuge at 1500rpm / 30min, and take interface layer cells, PBS Wash twice, suspend in serum-free GT-T561 medium, adjust cell concentration to 1×10 6 cells / mL.
[0028] (2) Preparation of super CIK killer cells
[0029] In the PBMC cells prepared on the same day, add , at 37°C, 5% CO 2 cultured in an incubator.
[0030] After 24 hours, add C...
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