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DNA (Deoxyribonucleic Acid) electrochemical sensor based on indigo carmine electrochemical polymer membrane and preparation method thereof

A technology of indigo carmine and chemical polymerization, applied in the direction of electrochemical variables of materials, etc., can solve problems such as unseen

Inactive Publication Date: 2013-02-06
MINNAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

It has been reported in the literature that a polyindigo carmine film was prepared by electrochemical polymerization, and horseradish peroxidase was fixed by electrostatic adsorption to construct a new type of enzyme electrode; there were also literatures that prepared a polypyrrole-indigo carmine composite membrane and used for the simultaneous determination of dopamine and ascorbic acid, but there is no report on the preparation of DNA electrochemical sensor based on indigo carmine electrochemical polymer membrane

Method used

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  • DNA (Deoxyribonucleic Acid) electrochemical sensor based on indigo carmine electrochemical polymer membrane and preparation method thereof
  • DNA (Deoxyribonucleic Acid) electrochemical sensor based on indigo carmine electrochemical polymer membrane and preparation method thereof
  • DNA (Deoxyribonucleic Acid) electrochemical sensor based on indigo carmine electrochemical polymer membrane and preparation method thereof

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Embodiment Construction

[0023] In order to further explain the technical solution of the present invention, the present invention will be described in detail below through specific examples. It should be noted that the electrochemical experiments in the present invention were carried out on a CHI650C electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.).

[0024] The invention relates to a DNA electrochemical sensor based on indigo carmine electrochemical polymer film, which is a three-electrode system sensor, which has a working electrode, an auxiliary electrode and a reference electrode, wherein the auxiliary electrode is a platinum electrode, and the reference electrode It is an Ag / AgCl electrode, and the working electrode is S1 / PIC / GCE based on a glassy carbon electrode, that is, the indigo carmine polymer film modified glass after covalently immobilizing the single-stranded probe DNA with the 5'-terminal modified amino group. carbon electrode.

[0025] The present invention also p...

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Abstract

The invention discloses a DNA (Deoxyribonucleic Acid) electrochemical sensor based on an indigo carmine electrochemical polymer membrane and a preparation method thereof. The DNA electrochemical sensor is a three-electrode system sensor with a working electrode, an auxiliary electrode and a reference electrode, wherein the working electrode is a glassy carbon electrode which is modified by the indigo carmine electrochemical polymer membrane after a 5'-terminal modified amino single-chain probe DNA is covalently fixed. The DNA electrochemical sensor has the characteristics of short response time, low limit of detection, excellent stability and excellent reproducibility.

Description

technical field [0001] The invention relates to the field of electrochemical sensors, in particular to a DNA electrochemical sensor based on an indigo carmine electrochemical polymer film and a preparation method thereof. Background technique [0002] DNA biosensors play an important role in gene sequence analysis, disease diagnosis, and environmental monitoring. Among them, electrochemical DNA biosensors have become a research field in the field of bioanalysis because of their simplicity, rapidity, sensitivity, low cost, and ease of miniaturization. hotspot. [0003] Electrochemical DNA biosensors can be roughly divided into two categories: electrochemical DNA hybridization biosensors and non-gene recognition electrochemical DNA sensors. The process of electrochemical DNA hybridization biosensors to measure DNA includes DNA probe fixation, hybridization process, There are 4 steps for the indication of hybridization and the detection of electrochemical signals. For DNA ele...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 汪庆祥张旋高凤
Owner MINNAN NORMAL UNIV
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