Kit for detecting antinuclear antibody spectrum related to autoimmune diseases (AIDs)

Abstract

The invention relates to a kit for detecting antinuclear antibody spectrum related to AIDs. The kit comprises a membrane strip, enzyme-labeled liquid, a substrate and concentrating and washing incubation liquid, wherein the membrane strip is composed of a carrier piece and an antigen band, a critical quality control band and a functional quality control line which are sequentially fixed on the carrier piece. The antigen band is composed of at least two of dsDNA, nucleosomes, SmD1, ribosome P0proteins, histones, U1snRNP, Ro / SS-A(52KD), Ro / SS-A(60KD), La / SS-B, Sc1-70, CENP-B, Jo-1, AMA-M2, PM-Sc1, PCNA, Mi-2 and Ku through independent marking to a nitrocellulose membrane or a nylon membrane. The kit is provided with the ingenious critical quality control band, one critical quality control band can play a role in interpreting two or even more detection bands (antigen bands), and result determination is simpler and more reliable.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit for diagnosing diseases, in particular to a kit for detecting antinuclear antibody spectrum related to autoimmune diseases. Background technique [0002] Autoimmunity is the reaction of the body's immune system to self-antigens, producing autoantibodies and self-responsive T cells. Under normal physiological conditions, autoimmunity can promptly remove aging and dead self-cells and maintain the stability of the internal environment. In some pathological states, self-responsive T cells and autoantibodies that directly and indirectly destroy self-tissues may be produced, and cause lesions and dysfunction of corresponding organ tissues, which are called autoimmune diseases (AID) ). Common autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, systemic sclerosis, multiple dermatitis / myositis, mixed connective tissue disease, hyperthyroidism, juvenile diabet...

AI Technical Summary

Problems solved by technology

However, it has the following disadvantages: it cannot be used as a basis for diagnosis; the judgment of the result requires rich experience; the significance of titer is greater than that of karyotype, but the judgment of titer is highly subjective; the sensitivity is low and the specificity is not high

[0009] 2. Most of the purified antigens used are extracted from natural tissues, and the lack of purity (≥90%) still affects the sensitivity and specificity of detection

[0010] 3. The background of the membrane strip is dark, sometimes darker than the positive strip, which affects the reliability of the result

[0011] 4. Different antigen bands have different widths and band intervals, which affect the accurate interpretation of the results

[0012] 5. Similar products, such as the kit disclosed in CN200810132304.8, do not have a critical quality control band, and the interpretation of the results requires an additional control film strip, which increases the cost of the experiment. May cause false positive or false negative

At present, some related products also have critical quality control bands, but one quality control band can only interpret the results of one detection band or item. At present, there is no critical quality control band for two or more bands at the same time. Application of Interpretation

Images