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Culture medium for screening biological phosphorus removing microorganisms

A technology of biological phosphorus removal and culture medium, applied in the direction of microorganisms, separation of microorganisms, methods based on microorganisms, etc., can solve the problems of unsuitable culture medium, small number of microbial strains of biological phosphorus removal, poor pertinence, etc., and achieve a small number of miscellaneous bacteria , reasonable nutritional content, targeted effects

Inactive Publication Date: 2013-02-27
SHENYANG JIANZHU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems that the existing methods for screening biological phosphorus removal microorganisms are poorly targeted, the number of strains of biological phosphorus removal microorganisms is small and the medium used is not suitable for the growth of biological phosphorus removal microorganisms

Method used

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specific Embodiment approach 1

[0007] Specific embodiment 1: In this embodiment, the medium for screening biological phosphorus removal microorganisms is four mediums used for screening by biological phosphorus removal microorganisms in sequence, and the four mediums are respectively anaerobic enrichment medium according to the order used for screening , aerobic enrichment medium, solid medium and liquid medium; wherein each liter of anaerobic enrichment medium is composed of 0.3~0.5g of NaAc, 270~290mg of (NH 4 ) 2 SO 4 , 27~29mg of CaCl 2 2H 2 O, 350~370mg of MgSO 4 ·7H 2 O, 0.5~0.7mL trace element solution, 1.8~2.4mL vitamin solution and the remaining distilled water, the pH value of the anaerobic enrichment medium is 6.9~7.4; each liter of aerobic enrichment medium is composed of 270 ~290mg KH 2 PO 4 , 480~500mg of K 2 HPO 4 , 260~300mg (NH 4 ) 2 SO 4 , 26~30mg of CaCl 2 2H 2 O, 340~380mg of MgSO 4 ·7H 2 O, 0.5~1.2mL of trace element solution, 1.5~3mL of vitamin solution and the rest of ...

specific Embodiment approach 2

[0008] Specific embodiment two: the difference between this embodiment and specific embodiment one is the FeCl in the trace element solution in anaerobic enrichment medium, aerobic enrichment medium, solid medium and liquid medium 3 ·6H 2 O concentration is 2.5~3.5g / L, H 3 BO 3 The concentration is 0.25~0.35g / L, CuSO 4 ·5H 2 O concentration is 0.05~0.07g / L, KI concentration is 0.32~0.4g / L, MnCl 2 4H 2 O concentration is 0.2~0.28g / L, Na 2 MO 4 2H 2 The concentration of O is 0.1~0.14g / L, ZnSO 4 ·7H 2 The concentration of O is 0.2~0.28g / L and CoCl 2 ·6H 2 The concentration of O is 0.28~0.32g / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0009] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the concentration of vitamin solution in anaerobic enrichment medium, aerobic enrichment medium, solid medium and liquid medium is 80 ~ 120mg / L of Vitamin B 1 , vitamin B with a concentration of 80~120mg / L 2 , the concentration is 180~220mg / L vitamin B 6 , vitamin B with a concentration of 1.8~2.2g / L 12 , folic acid with a concentration of 36~44mg / L, niacin with a concentration of 80~120mg / L, calcium pantothenate with a concentration of 80~120mg / L, p-aminobenzoic acid with a concentration of 80~120mg / L and 36~120mg / L 44mg / L biotin composition. Others are the same as in the second embodiment.

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Abstract

The invention relates to a culture medium for screening biological phosphorus removing microorganisms, which relates to a culture medium for screening phosphorus removing microorganisms. The culture medium for screening biological phosphorus removing microorganisms solves the problem that current culture medium for screening is poor in pertinence, and the number of bacterial strain of microorganisms is small and the culture medium used is not suitable for growth of phosphorus removing microorganisms. The culture medium for screening is an anaerobic enriching culture medium, an aerobiotic enriching culture medium, a solid culture medium and a liquid culture medium. The method comprises the steps of: 1, preparing the culture medium; 2, taking sludge; 3, preparing the anaerobic enriching culture medium; 4, preparing the aerobiotic enriching culture medium; 6, preparing the anaerobic enriching culture medium; 7, circularly operating the steps 4-6 for 2-4 times; 8, diluting at equal gradient; 9, preparing the solid culture medium; 10, preparing the liquid culture medium; 11, repeating the steps 8-10 for 3-8 times; and 12, detecting. The culture medium provided by the invention is strong in pertinence, the number of phosphorus removing microorganisms is great, and the culture medium is suitable for growth of the phosphorus removing microorganisms.

Description

technical field [0001] The invention relates to a culture medium for screening phosphorus-removing microorganisms. Background technique [0002] The current method for screening biological phosphorus removal microorganisms is to place the sludge taken from the biological phosphorus removal system into a shake flask containing beef extract peptone medium for fermentation and culture, then dilute the cultured bacteria solution, and then spread it on solid Traditional culture medium is used to screen biological phosphorus removal microorganisms, but this method still has the following problems: 1. The method is poor in pertinence, so during the cultivation process, non-biological phosphorus removal microorganisms multiply and miscellaneous bacteria grow, resulting in the proportion of biological phosphorus removal microorganisms decline, the number of isolated biological phosphorus removal microorganisms is small, only accounting for about 15% of the total number of isolated st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/38C12R1/01
Inventor 亢涵
Owner SHENYANG JIANZHU UNIVERSITY
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