Neomycin sulfate fermentation method

A neomycin sulfate and fermenter technology, applied in the biological field, can solve problems such as unstable quality of peanut cakes, reduce the difficulty of fermentation process control and adjustment, reduce unqualified product returns, and improve supply reliability Effect

Inactive Publication Date: 2013-03-13
YICHANG SANXIA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for fermentation of neomycin sulfate, wh

Method used

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  • Neomycin sulfate fermentation method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] like figure 1 Shown, a kind of method of neomycin sulfate fermentation is made up of the following steps:

[0037] 1. Cultivation of inclined spores: the sand spores are sealed, dried, and stored at 0-4°C to obtain inclined spores;

[0038] 2. Cultivate the slant spore suspension: culture the slant spore at 28±2°C for 4 to 12 days to prepare the slant spore suspension;

[0039] 3. Seed tank cultivation: put the slant spore suspension into the seed culture tank for cultivation, and the mass content of peanut meal in the seed tank is 1% of the quality of the seed tank culture solution;

[0040] 4. Fermentation tank cultivation: send the culture solution cultivated in the seed tank into the fermentation culture tank for cultivation, and the culture solution in the fermentation tank does not contain peanut meal;

[0041] 5. Add 732 resin to the fermentation broth, dilute the concentration of the fermentation broth to below 13000u / ml, neutralize to pH 6.4-7.0 to prepare a ...

Embodiment 2

[0052] like figure 1 Shown, a kind of method of neomycin sulfate fermentation is made up of the following steps:

[0053] 1. Cultivation of inclined spores: the sand spores are sealed, dried, and stored at 0-4°C to obtain inclined spores;

[0054] 2. Cultivate the slant spore suspension: culture the slant spore at 28±2°C for 4 to 12 days to prepare the slant spore suspension;

[0055] 3. Seed tank cultivation: put the slant spore suspension into the seed culture tank for cultivation, and the mass content of peanut meal in the seed tank is 1.5% of the quality of the seed tank culture solution;

[0056] 4. Fermentation tank cultivation: send the culture solution cultivated in the seed tank into the fermentation culture tank for cultivation, and the mass content of peanut meal in the culture solution in the fermentation tank is 2% of the quality of the fermentation tank culture solution;

[0057] 5. Add 732 resin to the fermentation broth, dilute the concentration of the fermen...

Embodiment 3

[0068] like figure 1 Shown, a kind of method of neomycin sulfate fermentation is made up of the following steps:

[0069] 1. Cultivation of inclined spores: the sand spores are sealed, dried, and stored at 0-4°C to obtain inclined spores;

[0070] 2. Cultivate the slant spore suspension: culture the slant spore at 28±2°C for 4 to 12 days to prepare the slant spore suspension;

[0071] 3. Seed tank cultivation: put the slant spore suspension into the seed culture tank for cultivation, and the mass content of peanut meal in the seed tank is 2% of the quality of the seed tank culture solution;

[0072] 4. Fermentation tank cultivation: the culture solution cultivated in the seed tank is sent into the fermentation culture tank for cultivation, and the mass content of peanut meal in the culture solution in the fermentation tank is 4% of the quality of the fermentation tank culture solution;

[0073] 5. Add 732 resin to the fermentation broth, dilute the concentration of the ferme...

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Abstract

The invention relates to a neomycin sulfate fermentation method. The method is characterized by comprising the following steps of: (1) culturing bevel spores: preparing the bevel spores from sandy soil spores; (2) culturing a bevel spore suspension: culturing the bevel spores for 4-12 days at the temperature of 28+/-2 DEG C, thereby preparing the bevel spore suspension; (3) culturing in a seeding tank: putting the bevel spore suspension in the seeding tank for culturing, wherein the mass content of peanut meal in the seeding tank is 1-2% the mass of culture fluid in the seeding tank; (4) culturing in a fermentation tank: feeding the culture fluid cultured by the seeding tank to the fermentation tank for culturing, wherein the mass content of the peanut meal in culture fluid in the fermentation tank is 0-4% the mass of the culture fluid in the fermentation tank; and (5) diluting fermentation broth into diluted fermentation broth, thereby completing the fermentation step. The neomycin sulfate fermentation method provided by the invention has the advantages that the problem of quality instability of peanut cakes can be solved, and the fermentation production of neomycin is effectively guaranteed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a neomycin sulfate fermentation method. Background technique [0002] The raw materials used in the existing domestic neomycin fermentation are mainly grain processing raw materials, such as broken rice, peanut cake, soybean cake, etc. In order to improve the bioavailability of these raw materials, the above raw materials usually need to be crushed and sieved , to make rice flour, peanut flour and soybean flour. [0003] In order to save costs, at present, manufacturing enterprises have long used peanut cakes as raw materials for neomycin fermentation production. Peanut cakes are mainly the residues obtained after hot pressing or cold pressing of peanuts. Since peanut cakes are mostly produced in small farm workshops, there are Unstable quality, uneven quality, black peanut cakes due to lax processing control in small workshops (normal peanut cakes should be brown), the color of bla...

Claims

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Application Information

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IPC IPC(8): C12P19/52
Inventor 沈联兵周云李文豪何连明姚明高
Owner YICHANG SANXIA PHARMA
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