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Preparation method of steinernema carpocapsae

A technique for the production of Stirneri elegans and nematodes, which is applied in the field of preparation of Stirronia minor, which can solve the limitations of the high production cost of entomopathogenic nematodes, the stagnation of entomopathogenic nematode production scale in the pilot test and commercial small-scale production stages Problems such as the promotion and application of pest control to achieve the effect of extending the shelf life

Inactive Publication Date: 2013-03-20
北京市西山试验林场
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Problems solved by technology

[0004] However, the current large-scale reproduction technology and low-temperature storage technology make the production cost of entomopathogenic nematodes too high, which limits its full play on pest control and further promotion and application, and also makes the production scale of entomopathogenic nematodes stagnant at In the stage of pilot test and commercial small-scale production, the research on entomopathogenic nematode mass reproduction technology and normal temperature storage technology has important scientific significance and application value

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  • Preparation method of steinernema carpocapsae

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited to the examples. Any improvements and changes made to the present invention are within the protection scope of the present invention.

[0026] 1. Mass reproduction method of the small toritrix nematode

[0027] 1. Commonly used culture medium formula

[0028] Strain medium formula: nutrient agar 45g, water 1000ml;

[0029] Differentiation medium formula: nutrient agar 18g, bromothymol blue 0.01g, distilled water 400ml.

[0030] Solid medium: soybean powder 15%, flour 10%, egg yolk powder 1%, peptone 1%, yeast extract 1%, sponge 10%, water 62%.

[0031] 2. Preparation of nematode symbiotic strains

[0032] The nematode symbiont is a rod-shaped bacterium, and the nematode symbiont is a white colony on the above-mentioned culture medium plate, and the colony is irregular in the initial stage of growth, and has an aroma...

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Abstract

The invention provides a preparation method of steinernema carpocapsae. The steinernema carpocapsae belongs to steinernematidae of rhabditida of phasmida, and is one of the entomopathogenic nematodes which are most widely and potentially applied to biological control. Symbiotic bacteria exist in bodies of the entomopathogenic nematodes and enter an insect along with the nematodes to result in blood poisoning which causes the death of the insect; and the steinernema carpocapsae has a unique advantage and a good control effect on control of concealed pests such as borers and soil pests.

Description

technical field [0001] The invention relates to a preparation method of Steineria nematode, which is used for preventing and controlling concealed pests (such as borers and soil dwellers), and belongs to the field of biological control of forest pests. Background technique [0002] Boring and soil-dwelling hidden pests are important types of agricultural and forestry pests, and the severity of their damage has become increasingly prominent with the adjustment of agricultural structure. This kind of pest has hidden habitat and harmful parts, long damage cycle, stable growth once the population is established, and currently lacks effective control methods, so it has become an internationally recognized major pest species that is difficult to control. At present, some high-toxicity and high-residue chemical agents are mainly used for prevention and control in production, but it is often difficult to achieve ideal results due to hidden life characteristics. On the other hand, t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/28A01P7/04A01K67/033
Inventor 梁洪柱田会鹏陈倩李学燕
Owner 北京市西山试验林场
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