Tumor model capable of reflecting prostatic cancer to generate zone functional difference and establishment method

Abstract

The invention provides an establishment method of a tumor model capable of reflecting prostatic cancer to generate zone functional difference, which comprises the following steps: 1) using prostatic stroma cells, respectively extracting a peripheral zone normal tissue and a transformation zone normal tissue according to a Mcneal anatomical definition, and culturing primary stromal cells from the peripheral zone normal tissue and the transformation zone normal tissue, and identifying the component; 2) using a hormone independent insensitive humanized prostatic cancer cell line145, mixing stromal cells according to equicellular quantity and injecting into the skin of a naked mouse; and 3) after the tumor is formed, acquiring a specimen at same time point of largest tumor volume with less than 400mm<3> and then identifying. According to the invention, the tumor model is realized by a mode of culturing in a prostatic stroma epiderm transformation zone transformation zone al compound body, the clinic characteristics of promotion by the peripheral zone and tumor generation inhibition by the transformation zone can be embodied, and the tumor model provides powerful reference for researching a mechanism of the zone difference generated by prostatic cancer.

Description

technical field [0001] The invention relates to the technical field of biological sciences, in particular to a tumor model reflecting the difference in sexual function of prostate cancer and its establishment method. Background technique [0002] Prostate cancer (PCa) is the most common male malignancy in western countries, and it has also become one of the most common malignancies in my country. Many patients are at an advanced stage when they see a doctor, and they tend to become hormone-independent and relapse after castration treatment. The treatment of PCa has become a clinical difficulty. The vast majority of PCa occur in the peripheral zone of the prostate, and a very small number of tumors in the transition zone are also relatively low in malignancy. This difference in girdle and the high recurrence rate after castration are important clinical features of PCa, and they are also hot spots and difficulties in research. Some studies believe that there are different rel...

AI Technical Summary

Problems solved by technology

Generally speaking, the treatment effect of prostate cancer is not good, and it is necessary to do more in-depth research on the pathogenesis of prostate cancer, especially the zonal differences in the occurrence of prostate cancer

[0003] However, the zonal difference in human prostate cancer cannot be replicated or simulated in vitro or in animal models so far, not only because of the anatomical characteristics of the human prostate, but also because of the lack of effective research on this phenomenon

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