Method for extracting and separating tyrosinase inhibitor from gomphrena globosa
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A technology of tyrosinase and Qianrihong is applied in the field of extraction of tyrosinase inhibitors to achieve the effect of good tyrosinase inhibitory activity
Inactive Publication Date: 2013-04-17
SOUTH CHINA UNIV OF TECH
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Problems solved by technology
At present, coumarin, ferulic acid, and some flavonoids have been isolated from Amaranth, but
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Embodiment 1
[0026] Add 5g of dry amaranth to 500mL water and mix, extract at 80°C for 4h, the extraction stirring speed is 100r / min, after extraction, filter and separate, the filtrate is light red, concentrate and dry under reduced pressure at 60°C to obtain Amaranth crude extract, the dried crude extract of Amaranth was added to 50% (v / v) ethanol solution to dissolve and settle to 100mL, and the tyrosinase inhibitory activity was measured to be 33%.
[0027] Wherein, the test of tyrosinase inhibitory activity takes L-tyrosine as substrate, and adopts spectrophotometric method to measure the inhibitory rate of tyrosinase activity. For specific measurement methods, see the following documents:
[0028] 1. Yan Guilong, Liu Naisen. The effect of kudzu root extract on tyrosinase activity[J]. Natural Product Research and Development. 2009,21:96-98.
[0029] 2. Prasad KN, Yang B, Yang S. Identification of phenolic compounds and appraisal of antioxidant and antityrosinase activities from litch...
Embodiment 2
[0031] Add 5g of dried amaranth to 400mL of methanol and mix, extract at 20°C for 0.5h, the extraction stirring speed is 250r / min, after extraction, filter and separate, the filtrate is dark blue, after concentrating and drying under reduced pressure at 40°C Obtain the crude extract of Auranthus auricosa, add the dried crude extract to 50% (v / v) ethanol solution to dissolve and settle to 100mL, and the measured tyrosinase inhibitory activity is 40%.
Embodiment 3
[0033] Add 5g of dried Amaranth to 300mL of 55% (v / v) methanol aqueous solution and mix, extract at 30°C for 3h, and the stirring speed of extraction is 150r / min. After concentrating and drying under reduced pressure at ℃, the crude extract of Auranthus acanthus was obtained, and the obtained dried crude extract of A. %.
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Abstract
The invention discloses a method for extracting and separating a tyrosinase inhibitor from gomphrena globosa. The method comprises the following steps: extracting a gomphrena globosa crude extract with tyrosinase inhibiting activity from water, methanol or ethanol and mixed solutions thereof; obtaining an extraction part from the gomphrena globosa crude extract by adopting an organic solvent; and further refining from the extraction part to obtain a monomeric compound with the tyrosinase inhibiting activity by adopting column chromatography on silica gel, hydroxypropyl sephadex gel filtration chromatography, and octadecyl silane bonded silica gel column chromatography. According to the invention, corresponding tyrosinase inhibiting components are obtained by adjusting the extracting and separating process according to different requirements while the tyrosinase inhibiting components from gomphrena globosa are enriched. The gomphrena globosa crude extract extracted by adopting different solvents not only has better tyrosinase inhibiting activity, but also the solution is in different colors.
Description
technical field [0001] The invention relates to a method for extracting tyrosinase inhibitors, in particular to a method for extracting and separating tyrosinase inhibitors from amaranth. Background technique [0002] Tyrosinase is a key rate-limiting enzyme that catalyzes the synthesis of melanin and is widely present in organisms. It is closely related to the important physiological processes of organisms. Abnormal overexpression of its activity can lead to human pigmentation diseases (such as freckles) , chloasma, age spots, etc.) Tyrosinase inhibitors have been added to whitening cosmetics as important additives. At present, the main whitening cosmetic additives commonly used in the market are mercury-containing compounds, hydroquinone, kojic acid, etc., because mercury-containing compounds are toxic, hydroquinone is sensitizing, and kojic acid is potentially carcinogenic, so these compounds are used in commercial products. Has been less and less used and added. [00...
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