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Anti-cariogenic bacteria saccharide composition

A technology of composition and cariogenic bacteria, applied in antibacterial drugs, drug combinations, food science, etc., can solve problems that have not been confirmed by research, and achieve the effect of inhibiting the formation of dental plaque

Inactive Publication Date: 2013-05-01
深圳市聚特佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no studies have confirmed whether they have the ability to inhibit cariogenic bacteria, especially whether they still have the ability to inhibit cariogenic bacteria in the presence of cariogenic sugars

Method used

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  • Anti-cariogenic bacteria saccharide composition
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  • Anti-cariogenic bacteria saccharide composition

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0056] Preparation of bacterial suspension

[0057] Inoculate the recovered bacteria for 48 hours in TPY liquid medium, and culture them anaerobically at 37°C for 18 hours. After the smear test shows pure culture, centrifuge the bacteria solution at 4000r / min for 10 minutes at 4°C to collect the bacteria and wash them with sterile normal saline. Twice, adjust the bacterial suspension with absorbance at 540nm ABS=1.0 with sterile physiological saline, set aside [19] .

[0058] Preparation of sugar compositions with different weight ratios

[0059] First prepare TPY liquid medium containing 1% glucose, and adjust the pH to 7.40. Prepare mixed samples of ISO and IMO in different proportions according to Table 4, then add the samples to the TPY liquid medium containing 1% glucose to make the final concentration of each sample reach 150 mg / ml, and adjust the pH to 7.40 again.

[0060] Table 4 ISO and IMO groupings of different weight ratios

[0061]

[0062] data proce...

Embodiment 1

[0064] [Example 1] Effect of the sugar composition of the present invention on the growth of oral cariogenic bacteria

[0065] According to the above method, sugar compositions (groups 1 to 21) and blank controls containing ISO and IMO in various weight ratios were prepared, and each group of concentrations was parallelized to do 4 tubes, all according to the bacterial liquid and TPY liquid medium 1: 10 (V / V) Proportional inoculation of bacteria, anaerobic culture at 37°C for 48 hours, and then bacterial counts and final pH determination [25] . Using the method of counting bacteria, dilute each group of bacterial solution after 48 hours of culture (the dilution factor depends on the growth of the bacteria), draw an appropriate amount into the cell counting plate, place it for 5 minutes, observe and count under an optical microscope, According to the number of bacteria, the growth status of bacteria under different drug concentrations was examined, and the growth ability of e...

Embodiment 2

[0070] [Example 2] The effect of the sugar composition of the present invention on the acid production of oral cariogenic bacteria

[0071] Shake the sample after culturing for 48h evenly, take 1ml in a 5ml small beaker, measure the pH value of the sample with a planar composite electrode, and calculate the change value of pH ΔpH [ΔpH=initial pH-final pH], take each pH value in the blank control group The acid production capacity of the bacteria was used as a control. see results Figure 4 ~ Figure 6 .

[0072] Figure 4 Shows the effect of different formulations of sugar compositions on acid production by Streptococcus mutans. The acid production of group 1 containing only ISO was significantly lower than that of group 21 containing only IMO (P<0.001), and the acid production from group 2 to group 18 was significantly lower than that of group 21 (P<0.01). The acid production in Group 2 and Group 3 was significantly lower than that in Group 21 and had no significant differ...

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Abstract

The present invention relates to an anti-cariogenic bacteria saccharide composition, which comprises, by weight, 5-95% of isomaltulose and 5-95% of isomaltooligosaccharide. The saccharide composition provides a significant anti-cariogenic bacteria effect in the presence of cariogenic sugars, is an ideal component for food ingredients and additives, has a dental caries prevention / anti-dental caries function, and can be applicable for various foods and products.

Description

technical field [0001] The invention relates to the technical field of food and daily necessities, in particular to a sugar composition with the effect of resisting cariogenic bacteria. Background technique [0002] Dental caries, commonly known as "tooth decay" and "tooth decay", is a disease with a high incidence rate in humans. According to the WHO report [1] In most countries in Europe, North America, South America and Australia, the caries loss index (DMFT) of people aged 35-44 is higher than 13.9 (that is, the average number of carious teeth per person in the oral cavity is higher than 13.6); the global In most countries, the caries and missing filling index of 12-year-olds in permanent teeth is also higher than 1.2; at the same time, although the prevalence of caries in developed countries has a downward trend, the prevalence of dental caries in developing countries is on the rise. Therefore, the prevention of dental caries has always been the focus of attention in ...

Claims

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Application Information

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IPC IPC(8): A23L1/09A61K8/60A61K31/702A61Q11/00A61P1/02A61P31/04
Inventor 肖悦马国霞
Owner 深圳市聚特佳生物科技有限公司
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