Method for extracting tigogenin from squeezed juice of sisal residue

A technology of sisal saponin and sisal residue, which is applied in the direction of steroidal compounds and organic chemistry, can solve the problems of undeveloped utilization and achieve the effects of shortening the production cycle, less energy consumption and high purity

Inactive Publication Date: 2013-05-08
GUANGXI NANJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After processing and extracting sisal fibers, sisal has a lot of sisal juice, which has not yet been developed and utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Squeeze the fresh sisal residue left after processing and extracting sisal fibers to obtain sisal juice. Filter the sisal with a 200-mesh filter cloth to extract the juice, remove the coarse residue, and obtain the coarse residue-removed filtrate. Centrifuge (5000 rpm) filtrate with disc centrifuge to obtain a transparent solution containing sisalin. Allow the transparent liquid to ferment naturally for 12 hours at normal temperature (25° C.) in a fermentation tank to obtain a fermentation liquid containing partially enzymatically hydrolyzed sisalin from sugar chains. The sisal saponins are collected by centrifugation (7000 rpm) in a disc centrifuge to obtain the sisal paste. Add sisal paste and concentrated sulfuric acid (the final concentration of sulfuric acid is 2mol / L) in the reaction kettle, heat to 121°C, keep the temperature at 121°C for 2 hours, acidolyze the sisal saponin, and obtain the acid hydrolysis solution containing sisalin . Use calcium hydroxide to...

Embodiment 2

[0028] Squeeze the fresh sisal residue left after processing and extracting sisal fibers to obtain sisal juice. Filter the sisal with a 200-mesh filter cloth to extract the juice, remove the coarse residue, and obtain the coarse residue-removed filtrate. Centrifuge (6000 rpm) filtrate with a disc centrifuge to obtain a transparent solution containing sisalin. Allow the transparent liquid to ferment naturally for 8 hours at normal temperature (32° C.) in a fermentation tank to obtain a fermentation liquid containing sisal saponins partially enzymatically hydrolyzed by sugar chains. The sisal saponins are collected by centrifugation (7000 rpm) in a disc centrifuge to obtain the sisal paste. Add sisal paste and concentrated sulfuric acid (the final concentration of sulfuric acid is 2.5mol / L) into the reaction kettle, heat to 125°C, keep the temperature at 125°C for 2.5 hours, acidolyze the sisal saponin, and obtain the acid hydrolyzed sisalin-containing liquid. Use calcium hyd...

Embodiment 3

[0030] Squeeze the fresh sisal residue left after processing and extracting sisal fibers to obtain sisal juice. Filter the sisal with a 200-mesh filter cloth to extract the juice, remove the coarse residue, and obtain the coarse residue-removed filtrate. Centrifuge (7000 rpm) filtrate with disc centrifuge to obtain a transparent solution containing sisalin. Allow the transparent liquid to ferment naturally for 24 hours at normal temperature (18° C.) in a fermentation tank to obtain a fermentation liquid containing sisal saponin partially enzymatically hydrolyzed by sugar chains. The sisal saponins are collected by centrifugation (7000 rpm) in a disc centrifuge to obtain the sisal paste. Add sisal paste and concentrated sulfuric acid (the final concentration of sulfuric acid is 3mol / L) into the reaction kettle, heat to 130°C, keep the temperature at 130°C for 3 hours, acidolyze the sisal saponin, and obtain the acid hydrolysis solution containing sisalin . Calcium hydroxide ...

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PUM

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Abstract

The invention discloses a method for extracting tigogenin from squeezed juice of sisal residue. The method for extracting tigogenin from squeezed juice of sisal residue disclosed by the invention comprises the following steps: squeezing juice by squeezing fresh sisal residue to obtain squeezed juice of sisal; filtering by filtering the squeezed juice of sisal and removing coarse residue to obtain a coarse residue filter liquor; centrifuging by centrifuging the filter liquor to obtain a transparent liquor; fermenting by naturally fermenting the transparent liquor at normal temperatures to obtain a sisalagenin fermentation liquor; centrifuging by centrifuging the fermentation liquor to obtain a sisal paste; carrying out acidolysis by adding the sisal paste and concentrated sulfuric acid into a reaction kettle, heating and carrying out acidolysis on the sisalagenin; drying by neutralizing sulfuric acid in an acidolysis liquor by using calcium hydroxide, heating and drying in vacuum, and crushing to obtain powdery tigogenin crude product; and purifying by extracting the sisalagenin in crude saponin by using hot ethanol, filtering, concentrating and stewing in vacuum, centrifuging and collecting saponin crystals, heating and drying to obtain finished products of tigogenin. The tigogenin extracted by the method for extracting tigogenin from squeezed juice of sisal residue disclosed by the invention has the advantages of good quality, high purity, less energy consumption, simple process, high recovery rate, energy conservation, environmental friendliness and the like.

Description

technical field [0001] The invention relates to an extraction method, in particular to a method for extracting sisal saponin from sisal residue by squeezing juice. Background technique [0002] Sisal, aliased as sisal hemp, water silk hemp, agave hemp, etc., belongs to Agaveaceae, Agave genus, and contains sisal saponin (tigogenin) in the form of steroidal saponins. Its C3 and C26 positions are connected with sugar chains through saponin bonds. To prepare sisal saponin, the saponin bond must be hydrolyzed. The sisal juice and sisal residue obtained during fiber extraction contain various saponins, the main components of which are steroidal saponins that can be hydrolyzed into sisal saponins. Sisal saponin has anti-inflammatory, antipyretic, analgesic, immune-enhancing, blood sugar-lowering, anti-tumor, anti-cancer, anti-bacterial, blood-fat-lowering, and blood-pressure-lowering effects, and is widely used as a precursor for the synthesis of steroidal drugs. More than 120 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 彭胜良
Owner GUANGXI NANJIAN BIOTECH
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