Application of magnolol in preparation of antitumor drug

An anti-tumor drug, the technology of magnolol, applied in the application field of magnolol in the preparation of anti-tumor drugs, can solve the problems of weak anti-autooxidation ability and no anti-oxidation activity, and achieve inhibition of proliferation and tumor suppression Obvious effect

Inactive Publication Date: 2013-06-05
FUDAN UNIV
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Problems solved by technology

The result shows: magnolol is weaker in the anti-autooxid

Method used

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  • Application of magnolol in preparation of antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1MTS method measures the growth inhibitory effect of magnolol on liver cancer cells

[0041] Hep-G2 cells (purchased from the Chinese Academy of Sciences Cell Bank) were inoculated into 96-well plates at 3,500 cells / well, cultured for 24 hours to allow them to adhere to the wall, and then magnolol (purchased from the Shanghai Institute of Materia Medica, Chinese Academy of Sciences) was added to set up 6 concentration gradients. , with 3 replicate wells for each concentration. Cells at 37°C, 5% CO 2 After culturing for 72 hours under the same conditions, the culture medium was discarded, and the cell viability was measured with an MTS kit (Promega).

[0042] The test method is: wash the cells once with serum-free medium, and add the pre-prepared MTS chromogenic solution according to the amount of 100 μl / well (add 2ml of solution 1 and 100μl of solution 2 to 10ml of serum-free medium, and mix well). A well without cells was set as the background well to corr...

Embodiment 2

[0045] Example 2 The growth inhibitory effect of magnolol on human cervical cancer cells

[0046] Using cck-8 kit (Japan Dojin Chemical Research Institute) detection.

[0047] step:

[0048] 1) HELA cells (purchased from ATCC) were evenly seeded in a 96-well plate, with 3000 cells per well.

[0049] 2) Waiting to adhere to the wall, adding drugs after overnight, adding drugs (the concentrations of magnolol are 50, 16.67, 5.56, 1.85, and 0.62 μM respectively), and there are 3 replicate wells for each concentration.

[0050] 3) After culturing for 48 hours, replace the complete medium with a mixture of serum-free medium and CCK8 (10:1), and incubate in a 37°C incubator for 2 hours.

[0051] 4) Take 450nm as the measurement wavelength and 650nm as the reference wavelength, and measure the reading on a microplate reader.

[0052] Results: The IC50 value of magnolol on HELA cells was 15.89 μM.

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Abstract

The invention belongs to the chemical industry field and the medicine field, and relates to an application of magnolol in preparation of antitumor drugs. The invention provides the application of magnolol in preparation of antitumor drugs, and the tumor cells comprise liver cancer cells, and cervical cancer cells. The magnolol belongs to a natural product, has little toxic and side effect, high bioavailability, and stable properties, and has clinical application value. The micromolecular compound of the invention can be developed as a new antitumor drug or its auxiliary component, has obvious tumor suppression effect, is green and environment-friendly, and provides a new approach and means for treating and curing tumors.

Description

technical field [0001] The invention belongs to the fields of chemical industry and medicine, and relates to the application of magnolol in the preparation of antitumor drugs. Background technique [0002] Magnolia officinale is brown to white fine powder Magnolia bark extract, the antibacterial active ingredient in the traditional Chinese medicine Magnolia officinalis, has a special, long-lasting muscle relaxation effect and strong antibacterial effect, and can inhibit platelet aggregation. It is mainly used clinically as an antibacterial and antifungal drug. [0003] The basic parameters of magnolol are as follows: [0004] 【English name】Magnolol [0005] 【Alias】5',5-diallyl-2,2'-biphenyldiol [0006] 【Chemical name】, 6', 7, 12-Tetramethoxy-2, 2'-dimethyl-1-beta-berbaman [0007] 【Molecular formula and molecular weight】C18H1802, 266.32 [0008] 【CAS No.】528-43-8 [0009] [structural formula] [0010] [0011] The application of supercritical CO by Li Jiaxiao et a...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P35/00A61P1/16A61P15/00
Inventor 余龙刘祖龙胡立宏
Owner FUDAN UNIV
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