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Construction method and purpose of recombinant lactobacillus acidophilus expressing pig epidermal growth factors

An epidermal growth factor, Lactobacillus acidophilus technology, applied in the promotion of intestinal health of pigs, the construction of recombinant Lactobacillus acidophilus can solve problems such as ineffective repair, intestinal damage, etc., to repair intestinal loss , The effect of promoting animal growth and improving intestinal environment

Active Publication Date: 2013-06-12
HUBEI HAOHUA BIOTECH
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Problems solved by technology

[0006] The purpose of the present invention is to provide a construction method and application of recombinant Lactobacillus acidophilus, and to develop a method that can promote Recombinant Lactobacillus acidophilus for intestinal mucosal injury repair in piglets

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  • Construction method and purpose of recombinant lactobacillus acidophilus expressing pig epidermal growth factors
  • Construction method and purpose of recombinant lactobacillus acidophilus expressing pig epidermal growth factors
  • Construction method and purpose of recombinant lactobacillus acidophilus expressing pig epidermal growth factors

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specific Embodiment approach

[0022] See attached Figure 1-Figure 4 , the recombinant Lactobacillus acidophilus expressing porcine epidermal growth factor described in the present invention is constructed by the following method: according to the published porcine epidermal growth factor (egf) gene sequence and the characteristics of the expression vector plasmid, design a Primers: using porcine mandibular gland as material, extract mRNA, reverse transcribe to obtain cDNA as a template for PCR amplification, obtain a fragment containing the egf gene, and connect it with the expression vector plasmid pMG36e to obtain the recombinant plasmid pMG36e-egf. Transformation method The recombinant plasmid is electrotransformed into Lactobacillus acidophilus competent cells for expression. And feed animals with the Lactobacillus acidophilus of the recombinant porcine epidermal growth factor constructed, and observe its effect of preventing and treating diarrhea. Required experimental material of the present invent...

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Abstract

The invention discloses a construction method and a purpose of recombinant lactobacillus acidophilus expressing pig epidermal growth factors. Primers containing specific enzyme cutting sites are designed according to disclosed gene sequences and expression vector plasmid characteristics of the pig epidermal growth factors; pig lower jaw glandular tissues are taken as the material, a segment containing pig epidermal growth factor genes is obtained through a reverse transcription-polymerase chain reaction (RT-PCR), and the segment is connected with a lactobacillus expression vector plasmid pMG36e to obtain a recombinant plasmid pMG36e-egf, the recombinant plasmid is placed into lactobacillus acidophilus with an electrotransformation method to obtain the recombinant lactobacillus acidophilus expressing the pig epidermal growth factors, expression is performed under the induction of nisin, and correct expression of target genes is proved through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot. The recombinant lactobacillus acidophilus is used for preventing diarrhea occurrence, improving intestinal tract health of pigs and promoting growth performance.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and veterinary medicine, and in particular relates to a method for constructing recombinant Lactobacillus acidophilus expressing porcine epidermal growth factor and its application in promoting the health of pig intestines. Background technique [0002] At present, the pig industry has become an important industry related to the national economy and the people's livelihood. In pig production, piglets are the stage with the fastest growth and development, the highest feed utilization rate, and the greatest development potential. The quality of piglet feeding directly affects the production performance of pigs throughout the feeding period. However, the piglet stage is also the most vulnerable stage of viability and physiological function, and the mortality rate of piglets usually accounts for 70% of the mortality rate in the whole feeding period. The low survival rate and slow growth of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/74C12N1/21A23L1/16A23L1/18C12R1/23A23L7/109
Inventor 侯永清吴涛窦茂鑫赵迪丁斌鹰
Owner HUBEI HAOHUA BIOTECH
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