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MUC1 protein nucleic acid aptamer, complex, composition and purposes thereof

A technology of nucleic acid aptamers and complexes, applied in drug combinations, pharmaceutical formulations, recombinant DNA technology, etc.

Active Publication Date: 2013-06-19
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cytotoxic drugs are the most important components in chemotherapy, and no literature has reported whether MUC1 aptamers can directly carry cytotoxic drugs to tumor cells

Method used

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  • MUC1 protein nucleic acid aptamer, complex, composition and purposes thereof
  • MUC1 protein nucleic acid aptamer, complex, composition and purposes thereof
  • MUC1 protein nucleic acid aptamer, complex, composition and purposes thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Materials and methods

[0041] reagent

[0042] Single-stranded oligonucleotides were synthesized by Invitrogen. Peptides were purchased from Beijing Saibaisheng Company. BSA was purchased from Tianjin Haoyang Biotechnology Co., Ltd. Monodisperse magnetic beads, streptavidin-coated magnetic beads were purchased from Promega. EDC was purchased from Sigma.

[0043] cell line

[0044] Human lung cancer cells A549, human breast cancer cells MCF7, human liver cancer cells HepG2, and human normal liver cells L02 were purchased from the Cell Center of the Chinese Academy of Medical Sciences. Cells were cultured in DMEM high glucose medium containing 10% FBS, conventional concentrations of penicillin and streptomycin (manufacturer: Beijing Yingxin Sunshine Biotechnology Co., Ltd., product number: 07-021, trade name: DMEM high glucose medium). cells at 37°C, 5% CO 2 Cultivated in an incubator. The cells used in all experiments were cells in logarithmic growth phase. ...

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PUM

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Abstract

The invention relates to an MUC1 protein nucleic acid aptamer, a complex, a composition and purposes thereof. The invention particularly relates to a nucleic acid aptamer specifically combined with an MUC1 protein, and the sequence of the aptamer is shown as SEQ ID NO.9. The complex is formed by the fact that the MUC1 protein is specifically combined with the nucleic acid aptamer and chemotherapy drugs, or the MUC1 protein, the nucleic acid aptamer and the chemotherapy drugs are wrapped in a nanometer particle. The composition comprises the complex. The nucleic acid aptamer, the complex and the composition have the purpose of tumor resistance and the purpose of tumor targeted radiography.

Description

technical field [0001] The present invention relates to MUCl protein nucleic acid aptamers, complexes, compositions and uses thereof. Background technique [0002] Chemotherapy is the most important way to treat tumors, especially for metastatic tumors in advanced stage. However, the side effects of cytotoxic drugs often limit the efficacy of chemotherapy. The main problem with cytotoxic drugs is that they can damage both tumor cells and normal tissues, causing severe side effects and limiting the intensity and duration of chemotherapy. Therefore, it is difficult for cytotoxic drugs to remove tumor cells in the body, which leads to the failure of clinical treatment and poor prognosis of patients. Based on this, new approaches are needed to reduce the toxicity of chemotherapeutic drugs. Targeted therapy is one way to achieve this goal. Targeted therapy means that targeted molecules carry drugs to the tumor site specifically, so that the drugs are more enriched in tumor ti...

Claims

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Application Information

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IPC IPC(8): C12N15/115A61K47/48A61K47/26A61K49/10A61P35/00
Inventor 杨先达胡燕
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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