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Kit and operation method thereof

A kit and microplate technology, applied in the field of immunoassay, can solve the problems of statistical error, enzyme-linked immunosorbent assay technical error, operation error, etc., and achieve the effect of ensuring the preservation time

Inactive Publication Date: 2013-06-19
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] The quality control of the kit is a serious problem. There are mainly technical errors, operational errors and statistical errors in the specific operation of the enzyme-linked immunosorbent assay (ELISA)

Method used

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  • Kit and operation method thereof
  • Kit and operation method thereof
  • Kit and operation method thereof

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] The embodiments of the present invention determine different stabilizers and related processing methods by screening the stability of the relevant reagents of the ELISA kit, thereby ensuring the storage time of the kit.

[0025] In the embodiment of the present invention, the composition of the kit includes: a 96-well ELISA plate, 1 mL of progesterone standard (40 ng / mL) and 8 mL of hormone-free milk, 5 mL of enzyme marker, substrate A solution (100 times concentrated solution ) 150μL and substrate B solution 10mL, stop solution (2MH 2 SO 4 ) 5mL and washing solution (10 times concentrated...

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Abstract

The invention is applicable to the technical field of immunoassay and provides a kit and an operation method thereof. The kit comprises an elisa plate with 96 holes, 1mL of a pregnendione standard product, 8mL of hormone removing paste, 5mL of an enzyme marker, 150 micro-milliliters of a zymolyte solution A, 10mL of a zymolyte solution B, 5ml of stop buffer and 20mL of a scrubbing solution. According to the kit and the operation method, the stability of related reagents of the ELISA kit is selected, so that different stabilizers and related treatment methods are determined, and further, the storage time of the kit is ensured.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to a kit and an operation method. Background technique [0002] At present, the pregnancy detection of dairy cows in our country still mainly relies on rectal examination, which has caused some artificial pregnancy abortions. Progesterone is a steroidal hormone, which is often used as an indicator of ovarian activity. In dairy cow production, the pregnancy, ovarian function and reproductive status of dairy cows can be detected by monitoring the change of progesterone content in the body. An effective approach to disease. The detection of progesterone in dairy cow milk can avoid the stress on the cow itself and reduce the artificial influence on the reproduction of dairy cows. It is an effective way to measure the progesterone content of dairy cows. [0003] Compared with foreign countries, there is still a certain gap in domestic research on the detection of proges...

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Application Information

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IPC IPC(8): G01N33/543G01N33/535
Inventor 张洪友郑家三夏成吴凌杜晓燕徐闯高维明
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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