Method for detecting activity of antitumor drug on inhibiting adhesiveness between tumor cell and blood platelet

Abstract

The invention relates to the field of biological chemistry, and discloses a method for detecting activity of an antitumor drug on inhibiting the adhesiveness between a tumor cell and a blood platelet. The method comprises the following steps of: labeling the blood platelets through fluorescently-labeled probes; respectively hatching the blood platelets with tumor cells without being treated by the antitumor drug and the tumor cells treated by the antitumor drug; on a flow cytometry, detecting the fluorescence rate of the tumor cells by utilizing an FL1 channel, so as to obtain the fluorescence rate serving as a control group and the fluorescence rate serving as a drug treated group; and analyzing the significant difference between the fluorescent rate in the control group and the fluorescent rate in the drug treated group, so as to obtain the detection result. According to the method, the tumor cells in a suspension state and the non-activated blood platelets are adopted for detecting, the process is in line with the adhesiveness process of the real tumor cells and the blood platelets; the cell density is adjusted by counting the cells before carrying out an adhesiveness test, so that the consistency of the density among the cells treated by the drug and that of cells in a contrast group can be ensured; and the quantity of the cells is detected under the setting of flow type analysis software, and therefore, the consistency of the quantity of the cells detected in different pre-treating groups can be ensured, the influence of different pre-treatments to the quantity of the tumor cells is avoided, and the accuracy of the result is improved.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for detecting the activity of antitumor drugs to inhibit the adhesion of tumor cells and platelets. Background technique [0002] Tumor invasion and metastasis is a complex multi-step and multi-factor process, including tumor cells secreting enzymes to degrade the surrounding matrix, dissociate from the primary tumor, enter the blood circulation, survive in the blood stream and penetrate the blood vessel wall, and then form metastases a series of processes. Tumor cells inevitably interact with blood components in the blood circulation, while immune cells in the blood can clear tumor cells, but platelets can promote the metastasis of tumor cells. Studies have found that cancer patients are often accompanied by elevated platelets, and antiplatelet therapy can inhibit tumor metastasis. [0003] The role of the interaction between tumor cells and platelets in the process of tum...

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Problems solved by technology

[0005] However, this detection technique has certain limitations, which are mainly reflected in the following aspects: (1) It is impossible to simulate the real process of tumor cell and platelet adhesion, and the tumor cells are in an adherent state. It is in a suspended state, so this method cannot reflect the real adhesion process between tumor cells and platelets; (2) The experimental operation is complicated, and the cells need to be planted on the culture plate in advance, and the cells are grown to complete fusion, and the experimental cycle is long; (3) It is difficult to quantitatively detect Some anti-tumor drugs can kill tumor cells or prevent tumor cells from adhering to the wall, so the number of tumor cells in the drug treatment group and the control group is inconsistent, and it cannot truly reflect whether the effect is achieved by inhibiting the adhesion of tumor cells and platelets ; (4) Using radioactive isotopes for inspection, causing radiation damage and environmental pollution

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