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Application of mesenchymal stem cells in preparation of multiple sclerosis treatment medicines, and extraction method of mesenchymal stem cells

A technology of multiple sclerosis and stromal stem cells, applied in medicine, in the field of skin mesenchymal stem cells, can solve the problem of undiscovered immune regulation function of skin mesenchymal stem cells, achieve no immune rejection, promote regeneration , good curative effect

Active Publication Date: 2015-04-01
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the immunomodulatory function of skin mesenchymal stem cells has not been discovered so far

Method used

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  • Application of mesenchymal stem cells in preparation of multiple sclerosis treatment medicines, and extraction method of mesenchymal stem cells
  • Application of mesenchymal stem cells in preparation of multiple sclerosis treatment medicines, and extraction method of mesenchymal stem cells
  • Application of mesenchymal stem cells in preparation of multiple sclerosis treatment medicines, and extraction method of mesenchymal stem cells

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Experimental program
Comparison scheme
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Embodiment Construction

[0026] 1. Isolation of Mouse Dermal Mesenchymal Stem Cells

[0027] Take out the newborn mice about 3 days old, kill them by necking, disinfect with alcohol, cut the back skin and put it in 0.25% dispase enzyme, digest it overnight at 4°C, wash it with PBS solution for 3 times, peel off the epidermis, and place the dermis in 0.2% dispase After the digestion, add DNase I to digest for 10 to 15 minutes, wash it with PBS, blow it into a single cell suspension with a dropper, filter to remove large pieces of tissue and debris, and inoculate in a petri dish , the culture condition is DMEM (low glucose) + 10% fetal bovine serum, 37°C, 5% CO 2 . After culturing for 6 hours, discard the suspended cells gently, and continue culturing the adherent cells. After most of the bottom of the bottle is covered, trypsinize and routinely pass. Cells passed down to the third passage were taken for research.

[0028] 2. Identification of Mouse Dermal Mesenchymal Stem Cells

[0029] Dermal mese...

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Abstract

The invention aims to provide an application of mesenchymal stem cells in the preparation of multiple sclerosis treatment medicines. The mesenchymal stem cells can effectively inhibit the excess activated effect T cell immune-response in the central nervous system of a multiple sclerosis patient, induce the generation of regulative T cells having a specific function and differentiate to form new nerve cells, so the damaged nerve tissues are repaired, thereby the multiple sclerosis is treated. The skin-derived mesenchymal stem cells used in the invention have the advantages of easy obtaining, no immune repellency, high curative effect, difficult recurrence after disease heal, and small side effects.

Description

technical field [0001] The invention relates to the application of mesenchymal stem cells in the preparation of drugs with immune regulation function, especially the application of skin-derived mesenchymal stem cells (Skin-derived Mesenchymal Stem Cells) in the preparation of drugs for multiple sclerosis. Background technique [0002] Multiple sclerosis (multiple sclerosis) is a chronic, inflammatory, autoimmune disease that occurs in the central nervous system (brain and spinal cord), characterized by abnormal neurodegeneration in the central nervous system (neurodegeneration) triggered by T Cells, B cells, and antigen-presenting cells are mainly infiltrated with inflammatory cells, which leads to demyelination in multiple nerves, and in the process of myelin tissue repair, damage and harden along the axons. The exact cause of multiple sclerosis is still unclear. Comprehensive factors such as environmental factors (such as infection, trauma, etc.) and genetic factors determ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61K35/36A61P25/00A61P37/02A61K35/28
Inventor 王宏林
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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