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Method for supercritical CO2 extraction of beta-carotene in spirulina

A carotene and spirulina technology, which is applied in the production of bulk chemicals, organic chemistry, etc., can solve the problems of harsh production conditions, small output, and no patents and literatures for extracting beta-carotene have been found. Short time, avoids the effect of disintegration and destruction

Inactive Publication Date: 2013-07-17
YUNNAN BLUE DIAMOND BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial fermentation method has disadvantages such as harsh production conditions, small yield, and currently unable to be widely promoted.
[0009] The same patent and literature on the method of extracting β-carotene from spirulina have not yet been found

Method used

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  • Method for supercritical CO2 extraction of beta-carotene in spirulina
  • Method for supercritical CO2 extraction of beta-carotene in spirulina

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Embodiment 1

[0036] A supercritical CO 2 Method for extracting β-carotene in spirulina, including CO 2Gas cylinders, high-pressure pumps, temperature controllers, extraction kettles, separation kettles I, separation kettles II, glass balls, filter paper and other equipment, in supercritical CO 2 Add entrainer in extracting spirulina, it is characterized in that: described entrainer is entrainer A and entrainer B, is to use acetone as entrainer A and raw material to mix first and put into extraction kettle together, in extraction process again Add entrainer B for extraction.

[0037] The method of adding the entrainer is to select fresh high-quality spirulina algae powder, pass through an 80-mesh sieve as the raw material powder, use the entrainer A to mix the raw material powder uniformly to obtain the mixture A, and place the mixture A in a clean container for 10-20 minutes Standby; mix the glass balls with the mixture A to obtain the mixture B, the diameter of the glass balls is 0.5-1....

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Abstract

The invention relates to a method for supercritical CO2 extraction of beta-carotene in spirulina. Devices such as a CO2 gas bottle, a high pressure pump, a temperature controller, an extraction kettle, a separation kettle I, a separation kettle II, a glass ball, filter paper and the like are comprised. An entrainer is added to supercritical CO2 extraction of spirulina. The method is characterized in that the entrainer consists of an entrainer A and an entrainer B and the method comprises the following steps of: by using acetone as the entrainer A, mixing the material with the entrainer A and packaging into the extraction kettle; and further adding the entrainer B in the extraction process to extract. The extract obtained contains 0.3-0.9% of beta-carotene based in dry weight of spirulina powder, and the extraction yield is over 95%.

Description

technical field [0001] The invention belongs to the field of plant extraction, in particular to an extraction method for extracting useful components in spirulina. Background technique [0002] Spirulina ( spirulina ) is a class of lower plants belonging to the phylum Cyanophyta, Oscillatoriaceae. It is a dark blue filamentous microalgae, which can be observed under a high-power microscope. It is a slender spiral algal body composed of single cells and single rows, so it is called Spirulina. Spirulina is rich in protein, polysaccharide, β-carotene, γ-linolenic acid, chlorophyll and various vitamins and minerals. Among them, the content of β-carotene is about 15 times that of carrots. It has various health functions such as anti-cancer, improving immunity, and delaying aging. . β-carotene is V A The precursor substance of V A It is a free radical scavenger with excellent antioxidant properties. It is known as the "anti-cancer god" and has obvious effects in anti-cancer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCY02P20/54
Inventor 不公告发明人
Owner YUNNAN BLUE DIAMOND BIOLOGICAL TECH CO LTD
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