Free fatty acid determination reagent kit
A technology of free fatty acids and kits, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of measurement deviation and unscientific, and achieve high specificity sexual effect
Inactive Publication Date: 2013-07-17
SHAOXING SHENG KANG BIOLOGICAL TECH
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Problems solved by technology
Determining the content of free fatty acids has become very important, and the previous determinations on the market have too many deviations and are not scientific
Method used
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[0033] Serum Free Fatty Acid Determination Kit for clinical detection, the kit is composed of the following two reagents, wherein
[0034] Reagent 1:
[0035]
[0036] Reagent 2:
[0037]
[0038]
[0039] Determination of samples, using two-point endpoint method, temperature 37 ° C; dominant wavelength 546nm (different models of biochemical analyzers can be selected according to the principle of proximity); method: endpoint method, reagent sample ratio: reagent 1: reagent 2: S is 210:70 : 4
[0040]
[0041] Free fatty acid (mmol / L) = standard solution concentration (mmol / L) * ΔA sample / ΔA standard solution.
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Abstract
The invention relates to a free fatty acid determination reagent kit which comprises dual reagents. The reagent 1 comprises Good's buffer solution (pH(potential of hydrogen)=7.0), coenzyme A, ATP (adenosine triphosphate), acetylcoenzyme A synthetase, magnesium chloride (MgCL2), Trinder bound constituent, tween 80 and liquid biological preservative / Proclin 300, and the reagent 2 comprises Good's buffer solution (pH=7.0), acetylcoenzyme A oxidase (ACOD), peroxidase (POD), Trinder bound constituent, tween 80 and liquid biological preservative / Proclin 300. The free fatty acid determination reagent kit has the remarkable advantages that the reagent kit is directly used for an automatic biochemical analyzer and is simple; and the adopted automatically separated and purified acetylcoenzyme A synthetase and acetylcoenzyme A oxidase (ACOD) have high specificity on free fatty acid in serum.
Description
technical field [0001] The invention belongs to the field of medical 6840 in vitro diagnostic reagents, in particular to a free fatty acid determination kit. Background technique [0002] Free fatty acids, also called non-esterified fatty acids, are the hydrolysis products of fat in the human body. Elevated free fatty acids are seen in pathological states such as insulin resistance / type 2 diabetes, obesity, malignant disease, and metabolic syndrome, and contribute to cardiovascular disease. Determining the content of free fatty acids has become very important, and the previous determinations on the market have too many deviations and are not scientific. Contents of the invention [0003] The purpose of the present invention is to provide a determination kit with simpler composition, convenient use, and fast and accurate detection of free fatty acids. [0004] The present invention is achieved through the following technical solutions: [0005] The present invention prov...
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IPC IPC(8): G01N21/78
Inventor 石丽萍
Owner SHAOXING SHENG KANG BIOLOGICAL TECH