Method for rapidly identifying monoacetylmorphine and morphine in hair

A technology of monoacetylmorphine and hair, which is applied in the field of rapid identification of monoacetylmorphine and morphine in hair, can solve the problems of high cost, use of a large amount of liquid nitrogen, and long time-consuming freeze-grinding technology to achieve strong selectivity and high sensitivity Effect

Active Publication Date: 2015-03-25
ACADEMY OF FORENSIC SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the freezing and grinding technology takes a long time, usually 3 hours, and a large amount of liquid nitrogen is required for freezing, which is costly

Method used

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  • Method for rapidly identifying monoacetylmorphine and morphine in hair
  • Method for rapidly identifying monoacetylmorphine and morphine in hair
  • Method for rapidly identifying monoacetylmorphine and morphine in hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] About 100 hairs of the suspect were cut by sticking to the roots, and the total length was measured to be 15 cm. They were recorded and placed in paper bags, and stored at room temperature. Cut off 3 cm of hair at the root, wash with 10 mL of 0.1% sodium dodecyl sulfate, 10 mL of 0.1% dish soap, 10 mL of water, and 10 mL of acetone for 2 min. Let dry at room temperature. Cut the washed hair section into small sections about 1 mm long. Take the shredded hair sample, accurately weigh 50 mg, place it in a polyacrylamide plastic tube, add 0.6 mL of the extract, 50 ng of internal standard morphine-d3, and then add 0.5 g of glass beads, and seal the plastic tube well. Finely pulverize in an automatic cell pulverizer. The setting parameters of the automatic cell pulverizer are: shaking time 3 min, shaking frequency 5000 rpm. After micro-grinding, cool down, centrifuge, and take 200 μL of the supernatant for direct injection. The sample was subjected to the above liquid chr...

Embodiment 2

[0047] About 100 strands of the suspect's hair were clipped and cut, and the total length was measured to be 4.5 cm. They were recorded and placed in paper bags, and stored at room temperature. Cut off 3 cm of hair from the root, and wash with 10 mL of 0.1% sodium dodecyl sulfate, 10 mL of 0.1% dish soap, 10 mL of water, and 10 mL of acetone for 2 min. Let dry at room temperature. Cut the washed hair section into small sections about 1mm long. Take the shredded hair sample, accurately weigh 50mg, place it in a polyacrylamide plastic tube, add 0.6mL of the extract, 50ng of internal standard morphine-d3, and then add 0.5g of glass beads, seal the plastic tube well, and place Finely pulverize in an automatic cell disruptor. The setting parameters of the automatic cell pulverizer are: shaking time 3 min, shaking frequency 5000 rpm. After micro-grinding, cool down, centrifuge, and take 200 μL of the supernatant for direct injection. The sample was subjected to the above-mention...

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Abstract

The invention provides a method for rapidly identifying monoacetylmorphine and morphine in hair. The method sequentially comprises the following steps: 1, placing hair to be detected, an extract liquid, internal standard morphine-d3 and a billiard ball in a crusher, and carrying out micro-crushing treatment; 2, centrifuging a mixture obtained after the micro-crushing treatment; and 3, taking the supernatant of the centrifuged mixture, and carrying out liquid chromatography-tandem mass spectrometry analysis of the supernatant. The method for rapidly identifying monoacetylmorphine and morphine in hair can realize the simple and rapid separation and purification of monoacetylmorphine and morphine molecules in a complex biological matrix, has the advantages of strong specificity, strong selectivity and high sensitivity, can be used for detecting a practical drug addict hair sample, and can also be used for identifying and detecting heroin addict related cases in the forensic toxicological analysis field.

Description

technical field [0001] The invention relates to a rapid identification method, in particular to a method for applying micro-pulverization extraction technology and liquid chromatography tandem mass spectrometry technology in analyzing monoacetylmorphine and morphine in hair. Background technique [0002] According to the "2010 World Drug Report", the total number of heroin addicts in my country ranks first in the world, which is one of the serious social problems at present. Heroin is well absorbed by smoking and injection, and can penetrate the blood-brain barrier in a large amount and take effect quickly. After entering the human body, heroin is rapidly metabolized into 6-monoacetylmorphine under the action of blood lipase, and then further metabolized into morphine in the liver and combined with glucuronic acid. The metabolites of heroin in urine include 6-monoacetylmorphine, free morphine, morphine-3-glucuronic acid conjugates, morphine-6-glucuronic acid conjugates and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 沈敏施妍向平沈保华孙英英
Owner ACADEMY OF FORENSIC SCIENCE
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