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Removal of selenocyanate or selenite from aqueous solutions

A technology of aqueous solution and selenous acid, applied in water/sludge/sewage treatment, biological water/sewage treatment, water pollutants, etc.

Inactive Publication Date: 2013-07-17
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although selenite (ester / salt) is suitable for removal by conventional co-precipitation methods, the remaining two species are relatively recalcitrant to the most popular precipitation techniques

Method used

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  • Removal of selenocyanate or selenite from aqueous solutions
  • Removal of selenocyanate or selenite from aqueous solutions
  • Removal of selenocyanate or selenite from aqueous solutions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0158] Spectrophotometric microassay to quantify SeCN - : starting rate method

[0159] A catalytic spectrophotometric method was used to quantify selenocyanate (ester / salt) in an aqueous matrix. All stock solutions and buffers were prepared according to Table 1. The analytical results from the calibration (Table 2) demonstrate that SeCN- can be quantified by this particular assay as long as its levels are within the range of the calibration standards (0.494-4.935 ppm).

[0160] Table 1: Stock solutions and buffers

[0161]

[0162]

[0163] Calibration standards ranging from 0.494 to 4.935 ppm Se (as SeCN-) in 50 mM sodium acetate (NaAc) buffer were prepared using SeCN-stock 2. Reagents, buffers, standards, and samples were added to the wells of a standard 96-well microplate according to the steps detailed in Table 3. Four wells were allocated for each standard and 8 wells for each sample. An additional four wells were reserved on the plate for a reagent blan...

Embodiment 2

[0169] Enzymatic removal of selenocyanate (esters / salts) from aqueous solutions )

[0170] Prepare a total working volume of 200 ml in four 250 ml flasks using stock buffer, reagents, Millipure water and Myceliophthora thermophila laccase according to Table 4. A Teflon-coated stir bar was placed in each flask, and glass wool was used to stopper the neck of each flask. The flask was then placed on a magnetic stir manifold submerged in a 40°C water bath. Care was taken to ensure that the contents of the flask were below the water level in the water bath. Insertion of tubes into each flask enabled continuous oxygen sparging throughout the incubation period. Oxygen was bubbled through millipure water prior to sample introduction to minimize sample loss over time. While stirring the oxygen sparged samples, Myceliophthora thermophila laccase was added to flasks 3 and 4. After 1 and 300 minutes of incubation, 1 ml aliquots were removed from each flask and immediately centrifuge...

Embodiment 3

[0177] Enzymatic removal of selenocyanate ( ester / salt)

[0178] Prepare a total working volume of 200 ml in five 250 ml flasks using stock buffer, reagents, Millipure water and Myceliophthora thermophila laccase according to Table 6. A Teflon-coated stir bar was placed in each flask, and glass wool was used to stopper the neck of each flask. The flask was then placed on a magnetic stir manifold submerged in a 40°C water bath. Care was taken to ensure that the contents of the flask were below the water level in the water bath. Tubes were inserted into each flask to enable continuous oxygen or nitrogen sparging throughout the incubation period. Oxygen or nitrogen is bubbled through millipure water prior to sample introduction to minimize sample loss over time. Oxygen was bubbled into flasks 1-4 and nitrogen into flask 5. While stirring the sparged samples, Myceliophthora thermophila laccase was added to flasks 3, 4 and 5. After 300 minutes of incubation, two 50 ml aliquo...

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Abstract

The invention relates to a process for removing selenocyanate or selenite from industrial effluents (from hydrocarbon processing industry, acid water, flue gas desulpurization) by contacting the aqueous effluent with a phenol oxidizing enzyme ( peroxidase, laccase) and suitable oxidizing agent ( hydrogen peroxide, oxygen). The method results in formation of elemental selenium, a Se (VI) salt and / or organoselenium compounds, such as selenite and / or selenate. Phenol oxidizing enzyme is used for removing slenocyanate or selenite from an aqueous solution.

Description

technical field [0001] The present invention relates to the removal of selenium species such as selenocyanate and selenium oxyanion from aqueous solutions such as effluent or wastewater. Background technique [0002] Selenium is a metalloid element whose effects on the environment and health have been well documented. Selenium circulates naturally in the environment, however, its balance can be significantly disrupted and affected by human activities including mining, mineral processing, agriculture, petroleum refining, and coal-based power generation. As a result, selenium levels in surface and groundwater are rapidly gaining global attention because of the established link between certain selenium species and environmental damage, including abnormal bioaccumulation and reproduction in waterfowl and fish. To this end, industries that tend to generate significant levels of the most toxic species of selenium, namely selenocyanate, selenite and / or selenate, must adopt measur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F2101/106C02F2103/365C02F2103/18C02F3/348C02F2103/10C02F3/342C02F2101/18
Inventor G.C.德洛齐尔H.伦德
Owner NOVOZYMES AS
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