Seed selection method and breeding method of yellow-bud pimento cytoplasmic male sterile line

Abstract

The invention relates to a seed selection method and a breeding method of a yellow-bud pimento cytoplasmic male sterile line. A yellow-bud pimento cytoplasmic male sterile plant is used as a female parent and a green-bud pimento is used as a male parent to be hybridized to obtain a hybridized seed first generation F1; the F1 is self-crossed to obtain a self-crossed first generation F2; the F2 is sowed; a yellow-bud complete sterile plant AF2 is used as a female parent and a yellow-bud fertile plant BF2 is used as a male parent to be hybridized to obtain a sterile plant hybridized second generation AF3; the yellow-bud fertile plant BF2 is used as a female parent and the green-bud pimento is used as a male parent to be hybridized to obtain a sterile plant hybridized seed second generation BF3; the BF3 is self-crossed to obtain a self-crossed second generation BF4; an AF3 and a BF4 are sowed; a complete sterile plant seed second generation AF3 with a yellow bud mark and a sterile plant second generation BF4 are selected to be hybridized to obtain a sterile plant hybridized third generation AF4; the BF4 and the green-bud pimento are hybridized to obtain a sterile plant hybridized third generation BF5; the BF5 is self-crossed to obtain a self-crossed seed third generation; and the steps are repeated to obtain a yellow-bud pimento cytoplasmic male sterile line seed.

Description

technical field [0001] The invention relates to a method for selecting and breeding a cytoplasmic male sterile line of sweet pepper, in particular to a method for selecting and breeding a cytoplasmic male sterile line of bud yellow sweet pepper, and belongs to the technical field of plant cultivation. Background technique [0002] As far as the applicant knows, sweet peppers have obvious heterosis. At present, the ways to utilize the heterosis of sweet pepper include artificial emasculation interspecific hybridization, double-cross, three-cross and male sterile line seed production, and male sterile line seed production is divided into male sterile dual-purpose line hybrid production There are two types of seed production and cytoplasmic male sterile line. Cytoplasmic male sterility (CMS) of sweet pepper has the advantage of stable sterility, but it is necessary to remove 50% of the female parent of the male sterile line at the initial flowering stage when producing swe...

AI Technical Summary

Problems solved by technology

Cytoplasmic male sterility (CMS) of sweet pepper has the advantage of stable sterility, but it is necessary to remove 50% of the female parent of the male sterile line at the initial flowering stage when producing sweet pepper CMS seeds. Breeding plants, so the sowing area and transplanting area of ​​the female parent are correspondingly enlarged by 50%, which increases the cost of seed production and reduces the yield of seed production. risk

Compared with the manual detasseling of sweet peppers at the bud stage, manual detasseling of sweet peppers is more difficult to operate, and it is easy to damage the stigma, high cost, time-consuming, and difficult to guarantee purity. Therefore, most sweet (spicy) peppers in the world Experts in pepper breeding shifted the focus of research on heterosis in sweet (hot) peppers to cytoplasmic male sterile lines

The cytoplasmic male sterility of sweet pepper is easily affected by environmental factors such as temperature and light. Once affected by environmental factors, such as low temperature, less sunshine, drought, etc., the fertility of the sterile line in the field will change. After selfing, the seeds The purity of the seeds cannot be guaranteed, making it impossible for such seeds to enter the market for promotion

[0003] At present, most of the cytoplasmic male sterile lines are produced by natural hybridization in the open field. Due to the low yield of hybrid seed production, and some male sterile lines can produce a very small amount of pollen, they can self-fertilize under the action of insects to affect the purity of hybrids.

If artificial hybrid seed production is adopted, pollination at the bud stage can improve the purity of the hybrid and produce high-purity hybrid seeds that meet the requirements, but this method not only increases the workload of artificial assisted pollination, but also reduces the pollination efficiency, and the purity of the hybrid The identification work is also time-consuming and labor-intensive.

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