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Molecule marking method for gene locus for preventing mycosphaerella melonis of muskmelon

A molecular marker, vine blight technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of few researches on the utilization of antibiotics, and achieve the effect of convenient identification, clear selection target, and clear location.

Inactive Publication Date: 2010-07-07
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, the research on the genetic basis of resistance to scabies and the utilization of disease-resistant breeding are mostly concentrated on the earliest discovered resistance resource PI 140471, while there are few studies on the utilization of other resistance sources.

Method used

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  • Molecule marking method for gene locus for preventing mycosphaerella melonis of muskmelon
  • Molecule marking method for gene locus for preventing mycosphaerella melonis of muskmelon
  • Molecule marking method for gene locus for preventing mycosphaerella melonis of muskmelon

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Embodiment Construction

[0033] The implementation procedure of screening muskmelon blight resistance gene molecular marker of the present invention comprises:

[0034] (1) F 1 (See references for PI420145 and PI136170: Wolukau et al., 2007, Resistance to Gummy Stem Blight in Melon (Cucumis melo L.) Germplasm and Inheritance of Resistance from Plant Introductions157076, 420145, and 323498. HortScience42(2): 215-221 .), F 1 F 2 , while backcrossing produces BC 1 generation. F grown in the experimental farm of Nanjing Agricultural University in the spring of 2006 2 Generations and BC 1 generational segregation groups. It was artificially inoculated and the incidence of each individual plant was counted. The chi-square test was used to analyze the genetic law of the resistance to the blight. Analysis found that 63 strains of BC 1 In the backcross population, 29 strains were resistant and 37 strains were susceptible, and the segregation ratio was completely consistent with the single-gene segregat...

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Abstract

The invention relates to a method for labeling the molecule of anti-vine blight gene of melon, belonging to biologic technical field. Four AFLP molecule labels are interlocked with the anti-vine blight gene; wherein, labeling the EcoRI-TG / MseI-CTC200 as 200bp gershgorim band, the EcoRI-AT / MseI-CTG90 as 90bp gershgorim band, the EcoRI-TC / MseI-CAG60 as 60bp gershgorim band and the EcoRI-TG / MseI-CTA70 as 70bp gershgorim band. The interlocking spaces are 2.0 cM, 6.0 cM, 5.4 cM and 6.0 cM respectively. The invention can be used for checking whether the PI420145 and the descendable breed (series) comprise the gene or not, and has the advantages of forecasting the ability of anti-vine blight, increasing the selecting efficiency and accelerating the seed selecting process of excellent breed of anti-vine blight melon. The invention can also be used in identifying the purity of the breed of anti-vine blight melon.

Description

1. Technical field [0001] The invention discloses a molecular marker method for a melon blight resistance gene locus, and belongs to the field of biotechnology. Dedicated to the molecular marker-assisted selection of muskmelon blight resistance, the breeding of muskmelon blight-resistant varieties and the utilization of germplasm resources. 2. Technical background [0002] Melon is one of the top ten fruits in the world. Melon blight is one of the most serious global fungal diseases. Its occurrence often leads to devastating consequences. In addition to harming melons, the fungus also infects other cucurbit crops such as cucumbers, watermelons, pumpkins and zucchini, resulting in different degrees of production reduction. Therefore, it is an urgent task in the current muskmelon breeding to study and prevent muskmelon vine blight. Breeding disease-resistant varieties is one of the safest, most effective and cost-effective measures to control the damage of muskmelon blight...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈劲枫鸠瑟夫·瓦鲁考周晓慧李英张永兵娄群峰钱春桃
Owner NANJING AGRICULTURAL UNIVERSITY
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