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Application of phenytoin sodium in preparing anti-angiogenesis medicine

An anti-angiogenesis and phenytoin technology, applied in the field of medicine, can solve problems such as phenytoin that has not been seen yet, and achieve the effects of good development prospects, wide source of raw materials and high safety

Inactive Publication Date: 2013-08-21
HANGZHOU LEISUO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There are no relevant reports on the anti-angiogenesis activity of phenytoin

Method used

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  • Application of phenytoin sodium in preparing anti-angiogenesis medicine
  • Application of phenytoin sodium in preparing anti-angiogenesis medicine
  • Application of phenytoin sodium in preparing anti-angiogenesis medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Qualitative observation of the inhibitory effect of phenytoin on zebrafish intersegmental vessel (ISV) generation

[0039] Zebrafish:

[0040] The zebrafish used in this example is vascular transgenic green fluorescent zebrafish (a gene specifically expressed by zebrafish endothelial cells acts as a driver to drive the specific expression of green fluorescent protein in zebrafish vascular endothelial cells) （ figure 1 ） , Feeding and use standards are strictly in accordance with the requirements of the American Institutional Animal Care and Use Committee (IACUC).

[0041] Fish water:

[0042] Preparation method: Add 0.3g of Instant Ocean salts to 1L of reverse osmosis (RO) water.

[0043] Dimethyl sulfoxide (DMSO, analytically pure):

[0044] Purchased from Aladdin (Catalog #1095515, Lot #30573). 0.1% DMSO solution (negative control) Preparation: When in use, prepare a working solution with a concentration of 0.1% with fish farming water...

Embodiment 2

[0054] Example 2 Quantitative evaluation of the inhibitory effect of phenytoin on zebrafish intersegmental vessel (ISV) generation

[0055] The budding of zebrafish vascular endothelial cells starts at 20hpf after fertilization, forms the main intersegmental vascular network at about 30-31hpf, such as dorsal long axis vessels (DLAV) and intersegmental vessels (ISV), and basically forms a complete body axis at 48hpf Vascular network [27] , the intact intersegmental vessels (ISVs) are clearly visible at this time. The complete intersegmental vessel mainly refers to the segment of vessel connecting the dorsal aorta (DA) and the dorsal long axis vessel (DLAV), see figure 1 (48hpf vascular transgenic fluorescent zebrafish body intersegmental vessel model). There are 28 pairs of intact intersegmental vessels (ISVs) in 48hpf zebrafish. The experimental method is as follows:

[0056] (1) Experimental grouping and embryo treatment: 270 well-developed zebrafish embryos were ta...

Embodiment 3

[0065] Example 3 Qualitative observation of the inhibitory effect of phenytoin on the generation of zebrafish inferior intestinal vessels (SIV)

[0066]The zebrafish subintestinal vessel (SIV, subintestinal vessel) grows on both sides of the yolk sac, and its shape is like a basket. The length of the subintestinal vessel (SIV) extending downward from the ventral side of the somites is about 50-100 μm [15-16] . See Figure 4 (72hpf vascular transgenic fluorescent zebrafish subintestinal vascular model). The experimental method is as follows:

[0067] (1) Experimental grouping and embryo treatment: 45 well-developed zebrafish embryos were taken, and the embryo development phase was 48hpf (hour-postfertilization, hpf) after fertilization, and were randomly divided into 3 groups (negative control group, drug group, positive control group) group), and the number of embryos in each group was 15. During the operation, the embryos were evenly distributed to 48-well cell cultur...

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PUM

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Abstract

The invention relates to an application of phenytoin sodium in preparing an anti-angiogenesis medicine. Reports related to the anti-angiogenesis activity of phenytoin sodium are unavailable so far. The invention provides an application of phenytoin sodium in preparing an anti-angiogenesis medicine, an anti-tumor medicine and an anti-wet senile vision macular degeneration medicine. An in-vivo pharmacodynamic experiment adopting a zebra fish angiogenesis model proves that the phenytoin sodium can obviously inhibit the zebra fish angiogenesis and the growth of transplanted human cancer cells, and has a therapeutic effect on wet senile vision macular degeneration. Therefore, the phenytoin sodium can be used for preparing a medicine for resisting angiogenesis, tumor or wet senile vision macular degeneration.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of phenytoin in the preparation of anti-angiogenesis drugs. Background technique [0002] Angiogenesis is highly related to many major human diseases, such as malignant tumors, age-related macular degeneration (Age-related macular degeneration, AMD), atherosclerosis (Atherosclerosis), rheumatoid arthritis (Rheumatoid arthritis), diabetes Retinal degeneration (Diabetic retinopathy) and tumor metastasis (Tumor metastasis), etc. [1] . With the gradual increase of the aging population in China, these major diseases have seriously endangered the life and health of the people of our country. [0003] In 1971, Professor Judah Folkman of Harvard University proposed anti-tumor blood vessel therapy for the first time. He believed that the growth and spread of solid tumors depended on the formation of new blood vessels in the tumor and obtained nutrients through the new b...

Claims

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Application Information

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IPC IPC(8): A61K31/4166A61P35/00A61P27/02
Inventor 李春启朱晓宇
Owner HANGZHOU LEISUO PHARMA
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