Method for inducing tetraploid thin-peel melon

A thin-skinned muskmelon and tetraploid technology, which is applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problem of inducing tetraploid of thin-skinned muskmelon without sulfamethoxazole, and achieves reduction of induction cost and improvement of quality. , the effect of improving the induction efficiency

Inactive Publication Date: 2013-09-11
南通致豪现代农业科技发展有限公司 +1
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[0004] So far, there is no invention of usi

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  • Method for inducing tetraploid thin-peel melon
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Embodiment Construction

[0015] A method for inducing tetraploids of thin-skinned melons, comprising the following steps:

[0016] (1) Induction treatment: select conventional diploid thin-skinned melon varieties as induction materials, soak seeds to accelerate germination according to conventional methods, and sow them in plug trays. Drop 2ml of sulfamiazine with a concentration of 30-40 μmol / L on the absorbent cotton ball with a 5ml syringe, titrate once every day at 8-9 am and 5-6 pm, and treat continuously for 7 days. Pay attention to shading during chemical treatment, and cover the treated groups with sunshade nets to prevent chemical volatilization. After the treatment, remove the cotton ball, pour it with clean water, and wash away the treatment solution.

[0017] (2) Ploidy identification: Place the true leaves of the treated plants on a glass slide, take the lower epidermis of the true leaves with tweezers, and drop 1-2 drops of KI-I 2 , cover with a cover glass, observe the number of chlor...

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Abstract

The invention discloses a method for inducing a tetraploid thin-peel melon. The method comprises the following steps of: selecting the diploid thin-peel melon variety as an inducing material; at the flattening stage of seed leaves of the thin-peel melon, putting degreasing cotton balls on growing points, selecting oryzalin with the concentration being 30-40 mumol/L for treatment, dripping once at 8-9 o'clock in the morning 5-6 o'clock in the afternoon everyday, dripping 2ml every time and continuously treating for 7 days; and then carrying out ploidy identification. The method has the advantages that since the oryzalin is used for replacing colchicine, the poison to an operator is reduced, the induction cost is reduced and the induction efficiency is improved. Compared with the diploid thin-peel melon, the tetraploid thin-peel melon obtained by the method has the advantages that the nutritive organs such as roots, stems and leaves, the floral organ and the reproductive organs such as fruits show large characters, the fruits are thickened, the storage and transportation resistance is improved, the sugar content of the fruits is increased and the quality is improved.

Description

technical field [0001] The invention relates to a method for inducing tetraploids of thin-skinned melons, belonging to the technical field of plant breeding. Background technique [0002] Triploid seedless melons are obtained by crossing diploid and tetraploid. In order to obtain excellent triploid seedless melons, it is key to obtain tetraploid materials by inducing doubling of diploid melons. At present, the main inducer of tetraploid melon is colchicine, and colchicine is toxic to humans and animals, and it will pollute the environment when dripped into the soil. The cost of colchicine is high, which increases the induction cost, and the tetraploid induction rate is low. (≤5%). [0003] Amisulamide is a chromosome doubling agent that has been widely used in recent years. It is currently used in watermelon (Yan Zhihong et al., 2008), cucumber (Han Yike et al., 2006), miscanthus (Karen et al., 2003), calla lily (Nguyen et al., 2003) successfully induced doubling. Compar...

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Application Information

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IPC IPC(8): A01H1/08
Inventor 何林池王康朱镇杰魏小云
Owner 南通致豪现代农业科技发展有限公司
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