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P-hydroxy phenylalanine urine detection reagent and preparation method thereof

A technology of hydroxyphenylalanine and detection reagent, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem that the reliability of statistical results is not strong and not applicable. Tumor screening, small number of specimens, etc., to achieve the effect of quick response, obvious results, and simple operation

Inactive Publication Date: 2013-09-25
吉林生物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reagents provided by the above-mentioned prior art have certain effects in application, high specificity and sensitivity, but there are still deficiencies. The reagents provided by Chinese patent CN1096374 have a high false positive rate, greater than 10%, and are not applicable Due to the general screening of tumors, the number of EP341803 specimens tested is small, so the reliability of the statistical results is not strong

Method used

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  • P-hydroxy phenylalanine urine detection reagent and preparation method thereof
  • P-hydroxy phenylalanine urine detection reagent and preparation method thereof
  • P-hydroxy phenylalanine urine detection reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] ① Preparation of liquid A: add 18g of mercury sulfate to a 6mol / L sulfuric acid solution and stir to dissolve it, then dilute it with 6mol / L sulfuric acid solution to 100ml to obtain a colorless and transparent liquid for use;

[0055] ② Preparation of solution B: add 65g of mercurous nitrate to a 12mol / L nitric acid solution, dilute to 100ml with 12mol / L nitric acid solution after dissolution, to obtain a green liquid, and place it until the green color of the solution fades before use;

[0056] ③. Preparation of liquid C: Dissolve 3g of nickel sulfate in distilled water, and then dilute to 100ml with distilled water to obtain a blue-green liquid, ready for use;

[0057] ④ Preparation of liquid D: dissolve 2g of α-nitroso-β-naphthol in 50ml, 6mol / L nitric acid solution to obtain liquid D for use;

[0058] ⑤ Mixing: Put the four liquids A, B, C, D prepared in ①, ②, ③, and ④ in a container at a volume ratio of 10ml:12ml:2ml:0.5ml to obtain the target Product p-hydroxyphenylalani...

Embodiment 2

[0060] The preparation of the four liquids of A, B, C, D is the same as in Example 1. After the four liquids of A, B, C, D and the volume ratio are 12ml:15ml:3ml:0.7ml, they are mixed in a vessel. Get the target product.

Embodiment 3

[0062] The preparation of A, B, C, D and the four liquids is the same as in Example 1. Put the A, B, C, D and the four liquids in a vessel with a volume ratio of 15ml:18ml:4ml:1ml to obtain Target product.

[0063] In actual production, the ratio of the above-mentioned embodiment can be expanded.

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Abstract

The invention relates to an in-vitro detection reagent for clinical diagnosis, particularly a reagent for determining metabolic disorder in a human body, particularly a p-hydroxy phenylalanine urine detection reagent and a preparation method thereof. The urine detection reagent is prepared by respectively dissolving Hg2<+>, Hg<2+>, Ni<2+> and alpha-nitroso-beta-naphthol in corresponding acid solutions and proportionally mixing. The mercuric sulfate is dissolved in a sulfuric acid solution as a donor of Hg<2+>, the mercurous nitrate is dissolved in a nitric acid solution as a donor of Hg2<+>, the nickel sulfate is dissolved in distilled water as a donor of Ni<2+>, and the alpha-nitroso-beta-naphthol is dissolved in a nitric acid solution. The invention overcomes the defects in the prior art, and provides a new-formula metabolic disorder quick urinalysis reagent which has the advantages of high stability, high sensitivity and wide application range.

Description

Technical field [0001] The invention relates to an in vitro detection reagent for clinical diagnosis, in particular to a reagent for judging abnormal metabolism in the human body-p-hydroxyphenylalanine urine detection reagent and a preparation method thereof. Background technique [0002] Major metabolic diseases such as malignant tumors and diabetes caused by abnormal cell metabolism in the body have attracted more and more attention. The key to treating diseases caused by abnormal metabolism in the body lies in early detection, early diagnosis and early treatment. At present, the diagnosis methods of diseases caused by this mainly include physics, tissue cytology and biochemistry. For tumor diseases, physics and histology can often only find the middle and late stages, which is difficult to achieve the purpose of early detection, and most of the operations are complicated and expensive. [0003] Early diagnosis of metabolic diseases such as malignant tumors is the most critical ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/82
Inventor 张凤华
Owner 吉林生物研究院有限公司
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