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6-chlorobenzoxazolone degrading bacterium and application thereof

A technology of chlorobenzoxazolone and degrading bacteria, which is applied in the field of microorganisms and can solve the problems of biodegradation of 6‐chlorobenzoxazolone that have not been found yet

Inactive Publication Date: 2014-12-10
吴旭平 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 6-Chlorobenzoxazolone (chlorzoxazone) is an analgesic drug, used for various acute and chronic soft tissue (muscle, ligament) sprains, contusions, muscle soreness after exercise, muscle spasms and chronic For fasciitis, no reports of biodegradation of 6‐chlorobenzoxazolone have been found so far, so screening strains that can efficiently degrade 6‐chlorobenzoxazolone is expected to provide new ideas for the biological treatment of such waste drugs

Method used

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  • 6-chlorobenzoxazolone degrading bacterium and application thereof
  • 6-chlorobenzoxazolone degrading bacterium and application thereof
  • 6-chlorobenzoxazolone degrading bacterium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1D

[0032] Example 1 DL-3 screening

[0033] Take 5g soil sample (collected from Zhenjiang City) and place 100mL containing 10mg·L -1 6-Chlorobenzoxazolone enriched medium (g·L -1 : NH 4 NO 3 1.0g, KH 2 PO 4 0.5g, K 2 HPO 4 1.5g, NaCl1.0g, MgSO 4 ·7H 2 O0.1g, yeast juice 0.02g, 6-chlorobenzoxazolone 0.01g, pH7.0) at 30℃, 180r·min -1 Cultivate for 5d. An ultraviolet scanner was used to measure the degradation of 6-chlorobenzoxazolone in the enrichment solution, and it was found that after 6-chlorobenzoxazolone was degraded, it was connected to 20mg·L with 10% inoculum -1 In the 6-chlorobenzoxazolone medium, continue to enrich and determine the degradation, according to this method until the concentration of 6-chlorobenzoxazolone increases to 50mg·L -1 , And passed down 3 times. The 6-chlorobenzoxazolone enrichment solution was serially diluted and spread on the LB plate and 50mg·L -1 MSM tablet with 6-chlorobenzoxazolone as the sole carbon source (g·L -1 : NH 4 NO 3 1.0g, KH 2 PO 4 0.5...

Embodiment 2

[0037] A single colony of Acinetobacter sp.DL-3 (CCTCC M2013227) was inoculated in 3mL liquid LB medium (g·L -1 :Yeast powder 5.0, tryptone 10.0, NaCl10.0, pH7.0. ), 37℃, 180r·min -1 Cultivate for 12h, transfer to 50mL liquid LB medium at 37℃, 180r·min at 5% inoculum -1 Cultivate for 12h to make seed liquid. The final concentration of 6-chlorobenzoxazolone is 20mg·L -1 Inorganic salt medium (g·L -1 : NH 4 NO 3 1.0g, KH 2 PO 4 0.5g, K 2 HPO 4 1.5g, NaCl1.0g, MgSO 4 ·7H 2 O0.1g, 6-chlorobenzoxazolone 0.02g, pH7.0), 5% inoculum amount was inserted into OD600nm = 1.0 strain Acinetobacter sp.DL-3 (CCTCC M2013227) seed solution, at 30 ℃, 180r ·Min -1 Shaker culture, sampling every 2h to determine the concentration of 6-chlorobenzoxazolone and its products, strain DL-3 can degrade 20mg·L within 48h -1 The 6-chlorobenzoxazolone reached 95%.

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Abstract

The invention belongs to the field of microorganisms and relates to a 6-chlorobenzoxazolone degrading bacterium and an application thereof. A 6-chlorobenzoxazolone degrading bacterium Acinetobacter sp.DL-2 is preserved in Chine Center for Type Culture Collection with the preservation date of May 21rt, 2013 and the preservation number of CCTCC M2013227. The 6-chlorobenzoxazolone degrading bacterium can degrade more than 90% of 6-chlorobenzoxazolone with the concentration of 20mg / L in 48 hours, is hopeful to being applied to treatment on 6-chlorobenzoxazolone medicines abandoned by hospitals, and an alternative bacterium is provided for carrying out biodegradation treatment on abandoned medicines.

Description

Technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of 6-chlorobenzoxazolone degrading bacteria and applications thereof. Background technique [0002] At this stage, the centralized treatment of waste medicines is mainly handled by chemical methods, which have high treatment costs and complicated treatment methods, and it is difficult to avoid secondary pollutants. Biodegradation is a method that uses microorganisms to degrade drugs, which can degrade waste drugs into substances that are not harmful to the environment. Compared with the chemical treatment method, the biodegradation method has the advantages of simple operation and low cost. With the gradual strengthening of environmental awareness and the rapid development of microbial technology, biodegradation and centralized treatment of waste drugs will become an alternative to chemical treatment methods. [0003] 6‐Chlorobenzoxazolone (chlorzoxazone) is an analgesic drug used...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
Inventor 吴旭平杨永峰易永祥赵伟董维亮
Owner 吴旭平