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Novel method for inducing Chinese mitten crab triploids

A Chinese mitten crab, triploid technology, applied in fish farming, application, climate change adaptation, etc., can solve the problem of poor triploid stability, low overall induction rate, limited drug permeability and accuracy of spawning time Judgment and other issues to achieve the effect of increasing the success rate and effectiveness and making it simple

Active Publication Date: 2015-03-11
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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  • Description
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AI Technical Summary

Problems solved by technology

However, due to the oviposition habit of Eriocheir sinensis, which limits the permeability of drugs and the accurate judgment of oviposition time, the induction of triploids still has problems such as poor stability and low overall induction rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, batch induction of triploid of Chinese mitten crab

[0029] This embodiment proceeds according to the following steps:

[0030] (1) Temporary breeding of parent crabs: On November 3, 2012, 200 female and male crabs with complete appendages, vigorous vitality, and well-developed gonads were purchased, and the male and female crabs were raised separately in 2 freshwater and sandy bottoms. Clams were temporarily raised in a cement pond (10.5×3.5×1.2m) for 7 days, and fresh clam meat was fed every day, and the water was changed appropriately depending on the water quality;

[0031] (2) Domestication in batches: 50 female and 50 male crabs were selected each time, and the male and female crabs were raised separately in 2 indoor cement ponds (3.5×1.5×1.2m) covered with sand at the bottom, divided into 4 times within 1 day Raise the salinity from 0 to 18‰, and maintain the salinity for 3~4 days, feed fresh clam meat every day, and change the water appropriately ...

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Abstract

The invention relates to a novel method for inducing Chinese mitten crab triploids. The method includes the following steps of seawater domestication of parent crabs, marking of the parent crabs, determination of a drug-induced start moment based on morphological markers, determination and application of KCI inducing optimal parameters and taking of drug permeability increasing measures. By the adoption of the steps of limitation of seawater domestication time, marking of the parent crabs, determination of the drug-induced start moment based on the morphological markers, taking of the drug permeability increasing measures and the like, stability and effectiveness of inducing the Chinese mitten crab triploids are obviously improved, and a technical support is provided for Chinese mitten crab triploid seed large-scale cultivation.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a new method for inducing triploids of Chinese mitten crabs. Background technique [0002] Chinese mitten crab (commonly known as river crab, hairy crab) aquaculture is one of the pillar industries of my country's aquaculture industry, but its development has encountered serious problems such as miniaturization of commercial crabs and precocious puberty. As one of the important ways to improve quality, polyploidy breeding has been successfully carried out in many kinds of fish and shellfish, and some species have reached the scale of commercial production. Based on the advantages of triploid animals in growth and development and the special characteristics of gonad development, the seedling breeding of triploid crabs is expected to solve the current problems of precocious puberty, slow growth, and small size of commercial crabs, so as to breed large-sized river crabs , Impro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张成松相建海李富花崔朝霞万建军
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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