Use of alpha-ketoglutaric acid
The technology of ketoglutaric acid and grass carp is applied in the application field of alpha-ketoglutaric acid, which can solve the problems of poor resistance to ammonia nitrogen stress, low immunity, slow growth of grass carp, etc., achieve strong disease resistance and promote grass carp Growth, improve feed conversion efficiency
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Embodiment 1
[0015] Example 1: Effects of α-ketoglutarate on growth performance and blood biochemical indicators of grass carp
[0016] Test materials: α-ketoglutaric acid was purchased from Shanghai Haiqu Chemical Co., Ltd., a white fine crystalline powder with a purity of 98.5%.
[0017] Test site: Hunan Agricultural University aquaculture base.
[0018] Test feed and grouping: select 180 healthy grass carp with an average body weight of 35.66 grams, and randomly divide them into two treatment groups, namely the control group and the α-ketoglutaric acid addition group (the feed group of the present invention), and each treatment has 3 repetitions , each repeated 30 tails, fed the basal ration and the feed ration of the present invention respectively. Grass carp basal diet is formulated according to the nutritional standard of grass carp compound feed (SC / T 1024-1997), and its composition and nutritional level are shown in Table 1:
[0019] Table 1 Composition and nutritional level of b...
Embodiment 2
[0030] Example 2: Effect of α-ketoglutarate on grass carp ammonia nitrogen stress
[0031] Test material, place, test feed and grouping, feeding test method are uniformly described in embodiment 1. After the feeding experiment (60 days), 15 fish with close to average body weight were randomly selected from each treatment, and put into 4 CL modulated the total ammonia nitrogen concentration of 20 mg / L in water for acute ammonia nitrogen stress test, carefully observed and recorded the behavioral response and death of fish.
[0032] The results of the acute ammonia nitrogen stress test showed that grass carp fed the basal diet group were extremely restless in the ammonia nitrogen stress test. As the exposure time prolongs, the swimming slows down and is accompanied by sideways or rollover movements, the breathing speed slows down, the operculum opening and closing slowly, and hides or swims into the bottom after being frightened. After 3 hours, some fish began to stiffen and d...
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