Usage of molecular marker for identifying gender of gingo

A technology of ginkgo biloba and gender is applied in the field of using molecular markers to identify the gender of ginkgo biloba, which can solve the problems of not making up for the dominant marker characteristics of SCAR markers, not ruling out the instability of RAPD technology, and achieving the effect of reducing time and labor and protecting the urban environment.
CN103374568AActive Publication Date: 2013-10-30KERALA FOREST RES INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KERALA FOREST RES INST
Publication Date
2013-10-30

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Abstract

The invention relates to a molecular marker for identifying gemale plant and male plant of gingo, more particularl, the invention relates to a method for identifying gender of gingo. Compared to the usage of random primer, the method has increased stability, and compared to the dominant marker, which can provide limit information, the method is improved. According to the mehtod, the gingo male plant specificity SCAR-GBM primer and the exsisted atp1 primer of the mitochondrial DNA which is developed in the field are used at the same time for performing multiple PCR, wherein, the SCAR-GBM primer is based on the sequence development of RAPD segment which can display the heredity of gingo male plant.
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Description

field of invention

[0001] The present invention relates to sex identification of Ginkgo biloba (Gingko biloba) using a molecular marker, and more particularly to a molecular marker capable of distinguishing male and female plants of Ginkgo biloba and a multiplex polymerase chain reaction used in an identification method using the molecular marker (PCR) conditions. Background technique

[0002] Ginkgo trees have a long history and are known as living fossils on the earth, and are widely distributed all over the world. In Korea, ginkgo is mostly used as a street tree, and for harvesting its fruit, ginkgo. Ginkgo biloba is dioecious, consisting of a male plant to provide pollen for fruiting and a female plant for fruiting. However, when ginkgo is used as a street tree, its own unpleasant smell and fruit drop in the autumn harvest season usually impairs city appearance. Meanwhile, ginkgo farmers currently encounter problems in cultivating and developing only female ginkgo pla...

Claims

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