Reagent strip for detecting PCT (procalcitonin) and preparation method of reagent strip
A technology of detection area and immunochromatographic test paper, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low PCT level, and achieve the effect of simple operation, reduced workload and fast response
Inactive Publication Date: 2013-12-18
管义东
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However, PCT levels in these cases are usually lower than those in patients with bacterial lesions
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[0019] A. Coat each antibody detection line working solution and quality control line working solution on a nitrocellulose membrane, and dry the obtained nitrocellulose membrane at 36-38° C. for 8-12 hours to obtain a chromatographic membrane;
[0020] B, mouse anti-human PCT monoclonal antibody and colloidal gold are prepared into colloidal gold binding pad;
[0021] C. Paste the chromatographic membrane and colloidal gold binding pad obtained in steps A and B on the glue board in order according to the order of sample pad, colloidal gold binding pad, chromatographic membrane and water-absorbing pad to prepare the described detection PCT immunochromatographic test paper .
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The invention discloses an immunochromatographic reagent strip, and particularly relates to an immunochromatographic reagent strip for detecting PCT (procalcitonin) and a preparation method of the reagent strip. A detection line is arranged on immunochromatographic test paper, and is formed by coating of an antibody, the antibody is selected from a mouse anti-human PCT monoclonal antibody, and a quality control line is formed by coating of a sheep anti-mouse IgG polyclonal antibody; the mouse anti-human PCT monoclonal antibody and colloidal gold are prepared into a colloidal gold combined pad. The preparation method of the immunochromatographic reagent strip for detecting the PCT is characterized by comprising the following steps: A, coating a nitrocellulose membrane with a detection line working solution and a quality control line working solution of each antibody, drying the obtained nitrocellulose membrane at a temperature of 36-38 DEG C for 8-12 hours to obtain a chromatographic membrane; B, preparing the mouse anti-human PCT monoclonal antibody and the colloidal gold into the colloidal gold combined pad; and C, sequentially adhering a sample pad, the colloidal gold combined pad, the chromatographic membrane and a water absorbing pad on a rubber plate to prepare the immunochromatographic reagent strip for detecting the PCT.
Description
technical field [0001] The invention discloses an immunochromatographic test strip, in particular to an immunochromatographic test strip for detecting PCT and a preparation method thereof. Background technique [0002] Immunochromatography test paper is a method of using the chromatographic principle to flow the target detection molecules or components in the liquid to be tested to the test line (T line) and quality control line (C line) on the test strip. The change indicates that the test result is positive, otherwise it is negative. When the color of the quality control line changes, it means that the test result is valid, otherwise it is invalid. [0003] Immunochromatographic test strips are very convenient in detecting or diagnosing certain diseases, so they have been fully used in the detection or diagnosis of various diseases or symptoms. In general, the immunochromatographic test paper includes the following structures: sample pad, binding pad, chromatographic memb...
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IPC IPC(8): G01N33/577G01N33/558
Inventor 管义东
Owner 管义东
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