Method for identifying embryo abortion associated proteins of distant hybridization of chrysanthemum

A technology of distant hybridization and related proteins, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of difficult to find and control embryo-related genes, and achieve high repeatability.

Inactive Publication Date: 2013-12-25
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

There are usually thousands of genes that control the development of plant embryos, and it is d

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  • Method for identifying embryo abortion associated proteins of distant hybridization of chrysanthemum

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Embodiment Construction

[0025] The present invention will be further described in detail below in conjunction with specific embodiments.

[0026] The present invention provides a method for quickly identifying the differential proteins between the embryos of the genus Chrysanthemum and the closely related genera that develop normal embryos and aborted embryos after the distant hybridization and pollination. The specific implementation examples are as follows:

[0027] 1. Distant hybridization and sampling

[0028] 1.1 Distant hybridization

[0029] Take the cultivated small chrysanthemum'Yuhua Luoying' (2n=6x=54) as the female parent, select the well-developed flower buds, and emasculate when the tongue-like flowers just show their color, that is, remove all the inner-ring bisexual tubular florets and cut the tongue-like Flower petals until the stigma is visible (do not hurt the stigma), and strictly bagging. When the female parent’s stigma stretches out and splits to show an acute angle and secretes mucus ...

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Abstract

The invention discloses a method for identifying embryo abortion associated proteins of distant hybridization of chrysanthemum, and belongs to the field of chrysanthemum breeding and proteomics. The method comprises the steps of planting the small chrysanthemum as a female parent, taking wild chrysanthemum nankingense as a male parent for distant hybridization, cutting off pollinated inflorescences at different times after pollination, sampling ovaries with complete and plump morphological development and ovaries with abnormal and hollow morphological development respectively, immediately quick-freezing in liquid nitrogen after sampling, storing in a refrigerator at -80 DEG C, extracting proteins from samples by a TCA (Trichloroacetic Acid)/acetone precipitation method, quickly identifying differential proteins by an iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and mass-spectrometric technique, performing bioinformatic analysis, and obtaining the embryo abortion associated protein in the distant hybridization breeding of the chrysanthemum. The method can quickly and accurately identify the embryo differential proteins with different morphologies in different developmental stages during embryonic development of the chrysanthemum for the chrysanthemum and affinis wild species of the chrysanthemum, and a research foundation is provided for solving embryo abortion of the distant hybridization of the chrysanthemum.

Description

Technical field [0001] The invention relates to the field of chrysanthemum hybrid breeding and proteomics, and relates to a method for rapid detection and identification of differential proteins between normal and aborted embryos in different developmental stages of embryos after chrysanthemum distant hybridization, and specifically relates to a method for identifying chrysanthemum distant hybrid embryos. Methods of breeding related proteins. Background technique [0002] Chrysanthemum is native to China. It is one of the top ten traditional famous flowers in my country, the top four cut flowers in the world and the top ten potted flowers. It occupies a very important position in flower production. At present, there are thousands of chrysanthemum varieties in China, but most varieties with excellent ornamental characteristics have poor resistance, and the wild resources of most related species contain excellent traits that many cultivated chrysanthemum varieties lack. The method...

Claims

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Application Information

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IPC IPC(8): G01N30/89
Inventor 滕年军张凤姣黄至喆陈发棣房伟民蒋甲福管志勇陈素梅刘兆磊
Owner NANJING AGRICULTURAL UNIVERSITY
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