Female cynoglossus semilaevis specific expression gene CSW2 and applications thereof

Abstract

The invention relates to a female cynoglossus semilaevis specific protein CSW2, whose amino acid sequence is represented by the SEQ ID No.2; the sequence of the nucleotide, which encodes the female cynoglossus semilaevis specific protein CSW2, is represented by the SEQ ID No.1. The external recombinant protein of the CSW2 gene can prominently improve the aromatizing enzyme expression level of female cynoglossus semilaevis fry related genes, namely fox12 and P450, has a biological activity which can promote female-related gene expression, and at the same time has a biological activity that can reduce the expression level of male-related gene SOX9. The recombinant expression product of CSW2 can be used as a feed addictive, which has the function of promoting the growth of female gonad and has an effect of improving the female fish ratio. So the CSW2 gene provided by the invention has a potential application prospect on aspects of cynoglossus semilaevis gender control and improvement on the female fry ratio.

Description

technical field

[0001] The invention belongs to the technical field of screening and application of functional genes in aquatic biology technology, and in particular relates to the screening, cloning, in vitro recombinant expression and activity analysis of tongue sole female-specific expression gene CSW2 of tongue sole. Background technique

[0002] Cynoglossus semilaevis (Cynoglossus semilaevis) is a large bottom-dwelling fish with warm water in the coastal waters. Its meat is delicious and nutritious, and it is deeply loved by consumers. It is one of the leading fish in mariculture in my country. However, the growth rate of female and male individuals of semi-smooth tongue sole is very different, and the growth rate of females is 2-4 times faster than that of males. Due to the slow growth and small size of male individuals, the cost of culture of tongue sole was increased, the production of culture was reduced, and the promotion of seedlings of tongue sole and the develop...

Examples

Embodiment Construction

[0018] The method of the present invention will be further described below in conjunction with the accompanying drawings. However, the examples are for illustration only and are not limited thereto. For the experimental methods that do not indicate specific conditions in the following examples, generally, conventional conditions can be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook), or according to the manufacturer's suggestion conditional run.

[0019] 1. Cloning and sequence of the female-specific gene CSW2 in half-smooth tongue sole

[0020] Whole-genome sequencing was carried out on the female and male fishes of the half-smooth tongue sole, and through bioinformatics analysis, it was found that there was a specific gene CSW2 in the genome of the ZW female individual, and the female was cloned from the ovary of the half-smooth tongue sole by using the rapid amplification of cDNA ends (RACE) technology. ...

technical field

[0001] The invention belongs to the technical field of screening and application of functional genes in aquatic biology technology, and in particular relates to the screening, cloning, in vitro recombinant expression and activity analysis of tongue sole female-specific expression gene CSW2 of tongue sole. Background technique

[0002] Cynoglossus semilaevis (Cynoglossus semilaevis) is a large bottom-dwelling fish with warm water in the coastal waters. Its meat is delicious and nutritious, and it is deeply loved by consumers. It is one of the leading fish in mariculture in my country. However, the growth rate of female and male individuals of semi-smooth tongue sole is very different, and the growth rate of females is 2-4 times faster than that of males. Due to the slow growth and small size of male individuals, the cost of culture of tongue sole was increased, the production of culture was reduced, and the promotion of seedlings of tongue sole and the develop...

Examples

Embodiment Construction

[0018] The method of the present invention will be further described below in conjunction with the accompanying drawings. However, the examples are for illustration only and are not limited thereto. For the experimental methods that do not indicate specific conditions in the following examples, generally, conventional conditions can be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook), or according to the manufacturer's suggestion conditional run.

[0019] 1. Cloning and sequence of the female-specific gene CSW2 in half-smooth tongue sole

[0020] Whole-genome sequencing was carried out on the female and male fishes of the half-smooth tongue sole, and through bioinformatics analysis, it was found that there was a specific gene CSW2 in the genome of the ZW female individual, and the female was cloned from the ovary of the half-smooth tongue sole by using the rapid amplification of cDNA ends (RACE) technology. ...

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