Nucleic acid suppression molecule for suppressing expression of brain eIF6 gene and screening method and application thereof

A gene expression and molecular technology, applied in gene therapy, genetic engineering, plant gene improvement, etc., can solve the problem of low eIF6 protein and achieve the effect of strong learning and spatial positioning ability

Inactive Publication Date: 2014-01-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

eIF6 can be detected in normal mucosal cells, but it is overexpressed in cancer cells, such as the upregulation of eIF6 in colorectal cancer[27], and it is especially obvious in lymph node metastases[28], so it is suggested that the high expression of eIF6 may be Involved in tumor proliferation and metastasis
[0019] It can be seen from the above that eIF6 plays an important role in ribosome synthesis, cytoskeleton, and tumor proliferation, differentiation and metastasis, but there are few related literatures on eIF6 protein, especially its relationship with learning and memory functions and its role in No effect on memory loss or memory impairment has been reported

Method used

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  • Nucleic acid suppression molecule for suppressing expression of brain eIF6 gene and screening method and application thereof
  • Nucleic acid suppression molecule for suppressing expression of brain eIF6 gene and screening method and application thereof
  • Nucleic acid suppression molecule for suppressing expression of brain eIF6 gene and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1. Open field test detection

[0084] The open field test, also known as the open box test, is a method to evaluate the autonomous behavior, exploration behavior and tension of experimental animals in a novel environment. The frequency and duration of certain behaviors of experimental animals in novel environments are used to reflect the autonomous behavior and exploratory behavior of experimental animals in unfamiliar environments, and the tension is reflected by the frequency of urination and defecation. The experimental device consists of two parts: an open field reaction box and an automatic data acquisition and processing system. In a quiet experimental environment, 12-13 8-10-week-old male eIF6 knockout mice (eIF6 single gene knockout mice imported from Italy, bred in SPF grade animal room; eIF6 knockout mice homozygous Lethal, heterozygous mRNA and protein expression are reduced by half) and wild-type mice with the same genetic background are lightly ...

Embodiment 2

[0085] Embodiment 2. Rotating Rod Type Fatigue Tester

[0086] The mouse rotary rod fatigue tester is designed according to the physiological characteristics of mice that are good at climbing but not good at running. The instrument can be used in the research of sports medicine, anti-fatigue drugs, muscle relaxant drugs, nervous system drugs, research on the changes of physiological indexes of animals under different exercise intensities, and research on the influence of drug toxicity on the exercise performance of experimental animals , 6 mice can be tested at the same time, the experimental conditions are consistent, and the experimental indicators are accurate. Put 12-13 8-10 week-old male eIF6 knockout mice and wild-type mice with the same genetic background on the rotarod, and insert the rotarod baffle. The mice were acclimatized for 20 minutes at a speed of 20 rpm / min, and the time the mice stayed on the rotarod at 30 rpm / min was recorded.

Embodiment 3

[0087] Example 3. Water maze test

[0088] The experiment lasted 4 days. Half an hour before the start of the experiment, the mice were placed in the room where the water maze was located to adapt to the environment. Each mouse was trained 4 times a day, and each time the water entry point was selected from four quadrants in a semi-random manner. The experimenter lifted the mouse with his hand to face the pool wall, and gently put it into the water. The mouse can find the platform within 90s, then record the time it needs to search and board the platform, ie the latency (latency). If the mouse failed to find the platform within 90s, the experimenter guided it to the platform by hand, and the latency was recorded as 90s. After the mouse boarded the platform, let it stay on the platform for 15 seconds, so that it can perform spatial learning and memory according to the reference objects of the four quadrants, and reduce the tension of the mouse. After each training session, d...

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Abstract

The invention provides a method for screening a nucleic acid suppression molecule for effectively suppressing the expression of a brain eIF6 gene from in-vitro cultured cells on the basis of the effect of eIF6 on negative regulation of memory and enhancement on glutamate receptor expression, the nucleic acid inhibition molecule screened by the method, and an application of the nucleic acid inhibition molecule in preventing and / or treating exercise-induced fatigue and / or forgetfulness and related diseases.

Description

technical field [0001] The present invention provides a method for screening nucleic acid inhibitory molecules that effectively inhibit the expression of the brain eIF6 gene, the nucleic acid inhibitory molecules screened by the method, and the nucleic acid inhibitory molecules for preventing and / or treating exercise-induced fatigue and / or memory loss and their related diseases. Background technique [0002] 1. Exercise fatigue [0003] Exercise-induced fatigue mainly refers to the phenomenon of decreased physical ability caused by exercise. In 1982, the Fifth International Sports Biochemical Conference unified the definition of fatigue, and exercise-induced fatigue is "the body's physiological process cannot continue its function at a certain level or cannot maintain the predetermined exercise intensity with various organs". [0004] At present, according to the location where fatigue occurs, it is customary to divide sports fatigue into two parts: central fatigue and per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113C12N15/86C12N15/861C12N15/867G01N33/68A61K48/00A61P1/16A61P3/10A61P5/14A61P9/10A61P25/00A61P25/08A61P25/14A61P25/16A61P25/18A61P25/20A61P25/24A61P25/28A61P35/00A61P39/02A61P39/06
CPCC12Q1/6883A61K31/713C12N15/113C12N2310/14C12Q2600/136
Inventor 崔艳艳吴军罗高兴桂莉谭江琳贺伟峰
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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