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Cordyceps militaris culture medium and application thereof

A technology of Cordyceps militaris and culture medium, applied to the application of Cordyceps militaris culture medium in the cultivation of Cordyceps militaris, the field of Cordyceps militaris culture medium, can solve the problems such as limited output of Cordyceps militaris, uneven quality, unsatisfactory effect and the like

Active Publication Date: 2014-01-29
TAIZHOU KANGJING BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, the output of artificially cultivated Cordyceps militaris is limited, its nutrient content is low, and its quality is uneven, which cannot meet people's needs.
Especially in terms of anti-tumor effect, it is often used to extract its active ingredients to make medicines for anti-tumor and other aspects of health care. It is not very ideal to directly use Cordyceps militaris to make medicines for anti-tumor effects

Method used

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  • Cordyceps militaris culture medium and application thereof
  • Cordyceps militaris culture medium and application thereof
  • Cordyceps militaris culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of Cordyceps militaris culture medium

[0022] Cordyceps militaris medium is an aqueous solution, which consists of dried silkworm chrysalis powder, rice flour, KH 2 PO 4 (potassium dihydrogen phosphate), NaH 2 PO 4 (sodium dihydrogen phosphate) and beef extract.

[0023] Take the following ingredients: 450g of dried silkworm chrysalis powder, 2.55kg of rice flour, 18g of potassium dihydrogen phosphate, 12g of sodium dihydrogen phosphate, 15g of beef extract; 2 PO4, NaH 2 Dissolve PO4 first, and mix thoroughly, then add dry silkworm chrysalis powder, rice flour, and beef extract and mix evenly to obtain a medium solution with a volume of 15L. ℃, 1.1Mpa sterilized for 30-40 minutes, and wait for it to condense naturally for later use.

Embodiment 2

[0024] Embodiment 2: the cultivation of Cordyceps militaris

[0025] Use the Cordyceps militaris culture medium that embodiment prepares to cultivate Cordyceps militaris, comprises the following steps:

[0026] (1) Inoculation: Inoculate the Cordyceps militaris strain into the culture bottle of the Cordyceps militaris medium prepared in Example 1 in the ultra-clean bench, and the Cordyceps militaris strain is Cordycepsmilitaris (L.) Link., liquid strain, the The density of the Cordyceps militaris bacterial classification is 3~5 bacterial balls / mL, 1ml of every culture bottle;

[0027] (2) Cultivate:

[0028] (a) Dark cultivation: cultivating in the dark for 3 to 5 days at a temperature of 22°C and a humidity of 75 to 85%, until the mycelium of Cordyceps militaris is covered with the surface of the Cordyceps militaris culture medium;

[0029] (b) The light culture used on the 5th to 20th day: 12 hours of sunlight during the day, the temperature is 20-22°C, the humidity is 80-...

Embodiment 3

[0040] Example 3: Inhibitory test of drugs made from Cordyceps militaris on Lewis lung cancer

[0041] The tumor-bearing mice mentioned in the examples are C57 inoculated with Lewis lung cancer. Mice C57 mice are ordinary-grade experimental animals, both male and female, purchased from the Experimental Animal Center of the Academy of Military Medical Sciences, and the experimental animal quality certificate number is SCXK-(Army) 2007-004. The lewis lung cancer, the source of the tumor, was preserved in our laboratory. Cyclophosphamide tablets, aluminum-plastic packaging, each containing 50mg of cyclophosphamide and 50mg of total saponins from ginseng stems and leaves, produced by Tianjin Jinshi Pharmaceutical Co., Ltd. Sodium chloride injection (normal saline), bottled, 500mL / bottle, Shandong Qidu Pharmaceutical Co., Ltd., production batch number 1D12052502, National Pharmaceutical Standard H37020764.

[0042] 1. Pulverization of fruiting bodies of Cordyceps militaris

[00...

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Abstract

The invention provides a cordyceps militaris culture medium. The cordyceps militaris culture medium comprises the following components: 30g / L of dry silkworm chrysalis meal, 170g / L of rice meal, 1.2g / L of potassium dihydrogen phosphate, 0.8g / L of sodium dihydrogen phosphate and 1g / L of beef extract. The cordyceps militaris culture medium is prepared, sealed, sterilized and cooled; then cordyceps militaris strains are inoculated, strain density is 3-5 pellets / mL, volume ratio of culture medium to strains is 50:1, culture, comprising dark culture and light culture, is carried out; fruiting bodies are harvested. Cordyceps militaris obtained by adopting the cordyceps militaris culture medium is prepared into a medicine, a C57 mouse inoculated with lewiss lung cancer is taken as an experimental subject, administration is carried out once everyday in the standard that administration dosage of cordyceps militaris in the medicine is 5g / kg, and after 10 days, the detected inhibition ratio on the lewiss lung cancer is 19.6%.

Description

technical field [0001] The invention relates to the technical field of Cordyceps militaris cultivation, in particular to a medium for Cordyceps militaris and the application of the medium for Cordyceps militaris in the cultivation of Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to the subphylum Ascomycota, Ergotaceae, and genus Cordyceps of the fungal kingdom. It is a medicinal fungus. Cordyceps militaris contains sugar, fat and protein, in addition, it also contains a variety of amino acids, trace elements and vitamins. A variety of physiologically active components in Cordyceps militaris have pharmacological effects such as anti-tumor, antibacterial, anti-oxidation, liver protection, and immunity enhancement. And its tumor-related active substances include cordycepin, polysaccharides, cordycepic acid, sterols, selenium, carotenoids, proteins, etc. [0003] However, at present, th...

Claims

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Application Information

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IPC IPC(8): C05G1/00
Inventor 陈剑清陈昊叶曼
Owner TAIZHOU KANGJING BIOLOGICAL SCI & TECH
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