Cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm
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A technology of cutting propagation and customs clearance rattan is applied in the field of asexual cutting propagation of traditional Chinese medicinal material clearance rattan, which can solve the problems of difficult and rapid rooting, perishable cuttings, narrow distribution area, etc. Effect
Inactive Publication Date: 2014-02-05
云南信通植物药业有限公司 +1
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Tongguan vines are mainly distributed in the middle and low altitude areas of Yunnan, Guangxi, Guizhou and other provinces. The distribution area is narrow. The plant vines are loose and porous, contain a lot of milk, and ev
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Embodiment 1
[0023] Test location: Caoba, Mengzi City, Honghe Prefecture, Yunnan Province.
[0024] (1) Picking time of cutting branches and selection of cutting branches
[0025] In March 2013, the three-year-old lignified customs clearance vines were used as cuttings;
[0026] (2) Seedbed establishment:
[0027] In Caoba, Mengzi City, Yunnan Province, at an altitude of 800m, a plastic greenhouse with a height of 2m was built on the flat red soil, and a seedbed with a height of 10cm and a width of 2m was built in the plastic greenhouse. The seedbed was made of peat, perlite, and river sand according to volume. The matrix is mixed at a ratio of 1:1:1. The thickness of the matrix is 15cm. A sunshade net with a light transmittance of 60% is built above the seedbed. The matrix is 500 times diluted with 50% carbendazim wettable powder and diluted 500 times. Potassium permanganate alternately sterilized once, watered thoroughly for later use;
[0028] (3) Pretreatment of cutting branch...
Embodiment 2
[0035] Test site: Jinping County Copper Factory, Honghe Prefecture, Yunnan Province.
[0036] (1) Picking time of cutting branches and selection of cutting branches
[0037] In May 2012, the 4-year-old lignified rattan cane was used as the cutting branch;
[0038] (2) Seedbed establishment:
[0039] At the Jinping County Copper Factory at an altitude of 1000m, select the red-yellow soil flat land at the foot of the mountain, build a plastic greenhouse with a height of 4m, and build a seedbed with a height of 10cm and a width of 2m in the plastic greenhouse. The seedbed uses peat, perlite, and river sand in a volume ratio of 1: The matrix is mixed at a ratio of 1:1. The thickness of the matrix is 15cm. A sunshade net with a light transmittance of 80% is built above the seedbed. The matrix is made of 500 times of 50% carbendazim wettable powder and permanganate diluted 500 times. Potassium alternately sterilizes once, water thoroughly for later use;
[0040] (3) Pretrea...
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Abstract
The invention discloses a cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm. The method comprises the steps as follows: a vinyl house is constructed in a reddish yellow soil area with an altitude of 800 m-1,000 m; lignified Marsdenia tenocissima(Roxb)Wight et Arm stems with more than three years of growth is picked in March to May, soaked in a 300mg/L IBA (indole butyric acid) solution after disinfection and sterilization, and then inserted in a seedbed; a sun shading mesh is constructed above the seedbed; in a first week after cuttage, the matrix humidity is controlled to be 90%-95%, the daytime temperature is controlled to be 25 DEG C- 34 DEG C, and the nocturnal temperature is controlled to be 20 DEG C-24 DEG C; in a second week, the matrix humidity is controlled to be 80%-89%, the daytime temperature is controlled to be 30 DEG C- 35 DEG C, and the nocturnal temperature is controlled to be 25 DEG C-28 DEG C; from a third week, the matrix humidity is controlled to be 60%-70%, the daytime temperature and the nocturnal temperature are controlled within 20 DEG C-35 DEG C; during the cuttage, ventilation is performed for half an hour every morning and 5 hours every noon until acclimatization and transplant after rooting of cuttings. According to the method, the cutting wood rooting percentage of the Marsdenia tenocissima(Roxb)Wight et Arm can reach 85%-93%, and the transplant survival rate can be higher than 92%.
Description
technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a method for asexual cutting propagation of Chinese medicinal material Tengguan vine. Background technique [0002] Tongguan vine (Marsdenia tenocissima (Roxb) Wight et Arm) is a vine of the genus Milkweed in the Ascaridaceae family. Its stems, roots, and leaves have anti-inflammatory, heat-clearing and detoxifying effects. The C21 steroids contained in Tongguanteng have the ability to eradicate the mitotic protivity of tumor cells. The traditional Chinese medicine Xiaoaiping preparation prepared from it as the main raw material is clinically used to treat various malignant tumors with good curative effect. [0003] At present, Guanguan vine mainly relies on natural breeding and limited supply of wild resources. Due to its own biological characteristics of more flowers and less fruit, the natural fertility of plants is low. In addition, high-int...
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