Cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm

Abstract

The invention discloses a cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm. The method comprises the steps as follows: a vinyl house is constructed in a reddish yellow soil area with an altitude of 800 m-1,000 m; lignified Marsdenia tenocissima(Roxb)Wight et Arm stems with more than three years of growth is picked in March to May, soaked in a 300mg / L IBA (indole butyric acid) solution after disinfection and sterilization, and then inserted in a seedbed; a sun shading mesh is constructed above the seedbed; in a first week after cuttage, the matrix humidity is controlled to be 90%-95%, the daytime temperature is controlled to be 25 DEG C- 34 DEG C, and the nocturnal temperature is controlled to be 20 DEG C-24 DEG C; in a second week, the matrix humidity is controlled to be 80%-89%, the daytime temperature is controlled to be 30 DEG C- 35 DEG C, and the nocturnal temperature is controlled to be 25 DEG C-28 DEG C; from a third week, the matrix humidity is controlled to be 60%-70%, the daytime temperature and the nocturnal temperature are controlled within 20 DEG C-35 DEG C; during the cuttage, ventilation is performed for half an hour every morning and 5 hours every noon until acclimatization and transplant after rooting of cuttings. According to the method, the cutting wood rooting percentage of the Marsdenia tenocissima(Roxb)Wight et Arm can reach 85%-93%, and the transplant survival rate can be higher than 92%.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a method for asexual cutting propagation of Chinese medicinal material Tengguan vine. Background technique [0002] Tongguan vine (Marsdenia tenocissima (Roxb) Wight et Arm) is a vine of the genus Milkweed in the Ascaridaceae family. Its stems, roots, and leaves have anti-inflammatory, heat-clearing and detoxifying effects. The C21 steroids contained in Tongguanteng have the ability to eradicate the mitotic protivity of tumor cells. The traditional Chinese medicine Xiaoaiping preparation prepared from it as the main raw material is clinically used to treat various malignant tumors with good curative effect. [0003] At present, Guanguan vine mainly relies on natural breeding and limited supply of wild resources. Due to its own biological characteristics of more flowers and less fruit, the natural fertility of plants is low. In addition, high-int...

AI Technical Summary

Problems solved by technology

Tongguan vines are mainly distributed in the middle and low altitude areas of Yunnan, Guangxi, Guizhou and other provinces. The distribution area is narrow. The plant vines are loose and porous, contain a lot of milk, and evaporate and lose water quickly after harvesting. Therefore, according to the conventional plant cutting propagation method , the cuttings are easy to rot, it is difficult to root quickly or the rooting rate is extremely low

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