A method for producing multiple secondary products using tamarisk tissue culture system

Abstract

The invention belongs to the field of bioengineering, and in particular relates to a method for producing various secondary products by using a tamarisk tissue culture system. Inner Mongolia Tamarix twigs were used as explants, treated with different hormone combinations (two levels of NAA and four levels of 6‑BA) and five types of media (MS, 1 / 2MS, NT, B5 and WPM). The total phenol content in the treated Tamarix bushy seedling tissue can reach 136.84-353.58 mg / g (dry weight), the total triterpenoid content can reach 17.65-63.13 mg / g (dry weight), and the total flavonoid content can reach 11.10-18.92 mg / g g (dry weight). Under the conditions of different types of medium with additional hormones of 6-BA 1.0mg / L+NAA 0.05mg / L, the total phenolic production of Tamarix tissue culture seedlings within one growth cycle (30d) (growth dry weight mg×content mg / g ) was the highest in 1 / 2MS, up to 60.76mg; the yield of total triterpenoids was the highest in MS medium, up to 9.13mg; the yield of total flavonoids was higher in 1 / 2MS and MS medium, 3.25mg and 2.50mg respectively. The invention of this application has laid the foundation for the large-scale production of medicinal components of Tamarix tamarix through biological fermentation methods.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for producing various secondary products by using a tamarisk tissue culture system. Background technique [0002] Tamarix is ​​a traditional Chinese medicinal material in my country. There are many records in herbal medicines of past dynasties. Pharmacological experiments have proved that it has antibacterial, cough, and antipyretic effects. It is mainly used clinically for rashes, chronic bronchitis, rheumatoid arthritis, and skin itching for external use. etc. Recent studies have shown that the tender branches and leaves of Tamarix are rich in terpenoids, flavonoids and phenolic substances, many of which have strong anti-tumor activity. The secondary metabolites of Tamarix have broad prospects for the development of new pharmaceutical preparations. [0003] At present, the research on Tamarix mainly includes: the medicinal components and pharmacological activi...

AI Technical Summary

Problems solved by technology

There have been some reports on tissue culture and rapid propagation of Tamarix, but they mainly focus on tissue rapid propagation, and there are few reports on the synthesis of secondary products of Tamarix tissue culture