The invention belongs to the field of bioengineering and particularly relates to a method for increasing the contents of total triterpenoids,
oleanolic acid and flavonoids of white birch tufted seedlings by using
temperature treatment. The method comprises the steps of inoculating a
callus of white birch to a NT+2mg / L6-BA
solid culture medium so as to induce the white birch tufted seedlings, transferring the white birch tufted seedlings to a NT+0.5mg / L6-BA
solid culture medium after twice subculture, and screening to obtain the white birch tufted seedlings with the highest content of the total triterpenoids; inoculating the screened tufted seedlings into the same
liquid culture medium at the inoculum concentration of 5 g / 100ml, respectively carrying
out stress treatment by using the high temperature of 45 DEG C and the low temperature of 4 DEG C at the seventh day of shake culture, harvesting after 24-48h, and detecting the contents of the total triterpenoids, total flavonoids and
oleanolic acid of the tufted seedlings. Through the stress treatment of two temperatures, the content of the total triterpenoids can be increased by 49.20%-5.03 times compared with that of the control, and the highest content can reach 59.66 mg / g; the content of the
oleanolic acid is increased by 3.68-10.56 times compared with that of the control, and the highest content can reach 2.33 mg / g; the content of the total flavonoids is increased by 10%-38.37% compared with the control, and the highest content can reach 4.0 mg / g. The method disclosed by the invention is
pollution-free and is low in cost, strong in
operability and high in efficiency, and a novel technology for solving the problem of natural
plant drug source lack and for the industrial large-scale production of white-birch total
triterpenoid and oleanolic acid drugs is provided.