Method for increasing contents of total triterpenoids, oleanolic acid and flavonoids of white birch tufted seedlings by using temperature treatment

Abstract

The invention belongs to the field of bioengineering and particularly relates to a method for increasing the contents of total triterpenoids, oleanolic acid and flavonoids of white birch tufted seedlings by using temperature treatment. The method comprises the steps of inoculating a callus of white birch to a NT+2mg / L6-BA solid culture medium so as to induce the white birch tufted seedlings, transferring the white birch tufted seedlings to a NT+0.5mg / L6-BA solid culture medium after twice subculture, and screening to obtain the white birch tufted seedlings with the highest content of the total triterpenoids; inoculating the screened tufted seedlings into the same liquid culture medium at the inoculum concentration of 5 g / 100ml, respectively carrying out stress treatment by using the high temperature of 45 DEG C and the low temperature of 4 DEG C at the seventh day of shake culture, harvesting after 24-48h, and detecting the contents of the total triterpenoids, total flavonoids and oleanolic acid of the tufted seedlings. Through the stress treatment of two temperatures, the content of the total triterpenoids can be increased by 49.20%-5.03 times compared with that of the control, and the highest content can reach 59.66 mg / g; the content of the oleanolic acid is increased by 3.68-10.56 times compared with that of the control, and the highest content can reach 2.33 mg / g; the content of the total flavonoids is increased by 10%-38.37% compared with the control, and the highest content can reach 4.0 mg / g. The method disclosed by the invention is pollution-free and is low in cost, strong in operability and high in efficiency, and a novel technology for solving the problem of natural plant drug source lack and for the industrial large-scale production of white-birch total triterpenoid and oleanolic acid drugs is provided.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for increasing the contents of total triterpenoids, flavonoids and oleanolic acid in birch bush seedlings by temperature treatment. Background technique [0002] Betula triterpenes, as the most potential new pharmaceutical preparations, have broader application prospects. However, the currently used birch triterpenoids are mainly derived from birch bark and its artificial chemical synthesis. Factors such as small quantity, low chemical synthesis efficiency, and high cost are the main reasons that restrict the large-scale clinical application of birch triterpenes. [0003] Since the production of plant natural active substances by fermentation and cultivation of plant tissues or organs is not affected by natural conditions such as climate, diseases and insect pests, and does not occupy land, does not consume natural resources, has the advantages of short producti...

AI Technical Summary

Problems solved by technology

However, currently used birch triterpenoids are mainly derived from birch bark and its artificial chemical synthesis. Factors such as small quantity, low chemical synthesis efficiency, and high cost are the main reasons that restrict the large-scale clinical application of birch triterpenes.