Method for producing various secondary products by tamarix chinensis tissue culture system

Abstract

The invention belongs to the field of bioengineering and in particular relates to a method for producing various secondary products by a tamarix chinensis tissue culture system. The method comprises the following steps: taking Inner Mongolia tender tamarix chinensis branches as explants, and treating the explants with different hormone combinations (two horizontal NAA (naphthylacetic acid) and four horizontal 6-BA (6-benzylaminopurine)) and five types of culture mediums (MS, 1 / 2 MS, NT, B5 and WPM) respectively, wherein in tamarix chinensis cluster seedling tissues treated with different materials, the total phenol content can reach 136.84-353.58 mg / g (dry weight), the total triterpene content can reach 17.65-63.13 mg / g (dry weight), and the total flavone content can reach 11.10-18.92 mg / g (dry weight); under the condition that additional hormones are different types of culture mediums of 1.0 mg / L of 6-BA and 0.05 mg / L of NAA, in a growth cycle (30 days) of tamarix chinensis tissue culture seedlings, the total phenol yield (growth dry weight mg*content mg / g) is highest in the culture medium 1 / 2 MS and can reach 60.76 mg, the total triterpene yield is highest in the culture medium MS and can reach 9.13 mg, and the total flavone yields are higher in the culture mediums 1 / 2 MS and MS and are 3.25 mg and 2.50 mg respectively. The method lays a foundation for large-scale production of medicinal tamarix chinensis ingredients through a biological fermentation method.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for producing various secondary products by using a tamarisk tissue culture system. Background technique [0002] Tamarix is ​​a traditional Chinese medicinal material in my country. There are many records in herbal medicines of past dynasties. Pharmacological experiments have proved that it has antibacterial, cough, and antipyretic effects. It is mainly used clinically for rashes, chronic bronchitis, rheumatoid arthritis, and skin itching for external use. etc. Recent studies have shown that the tender branches and leaves of Tamarix are rich in terpenoids, flavonoids and phenolic substances, many of which have strong anti-tumor activity. The secondary metabolites of Tamarix have broad prospects for the development of new pharmaceutical preparations. [0003] At present, the research on Tamarix mainly includes: medicinal components and pharmacological activities...

AI Technical Summary

Problems solved by technology

There have been some reports on tissue culture and rapid propagation of Tamarix, but they mainly focus on tissue rapid propagation, and there are few reports on the synthesis of secondary products of Tamarix tissue culture