Three-dimensional culture hepatocyte device capable of simulating hepatic plate structure and culture method of hepatocyte
A technology for three-dimensional culture and hepatocytes, which is applied in the field of liver tissue engineering and bioartificial liver, can solve the problems of not really reproducing the culture of hepatocytes, the functional status of hepatocytes is very different from that of hepatocytes, and achieve the maintenance of the morphological vitality of hepatocytes , Promote the effect of liver cell function
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[0026] see figure 1 , figure 1 It is a perspective view of the structure of the liver cell three-dimensional culture device with imitation liver plate structure in this embodiment, as shown in figure 1 As shown, the device in this embodiment is a polydimethylsiloxane (PDMS) microfluidic chip with channels inside.
[0027] The channel includes a main channel and a branch channel communicating with the main channel, and the main channel includes a first main channel 11 , a second main channel 12 and a third main channel 13 which are sequentially connected and communicated in a straight line.
[0028] The end of the first main channel 11 far away from the second main channel 12, the connection between the first main channel 11 and the second main channel 12, and the connection between the second main channel 12 and the third main channel 13 all extend symmetrically to form two branches. aisle. Specifically, the upper and lower sides of the first main channel 11 away from the s...
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