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Ethanol-pullulan polysaccharide coupled fermentation method

A technology of pullulan polysaccharide and fermentation method, which is applied in the field of ethanol-pullulan polysaccharide coupling two-step fermentation, can solve the problems of inconvenient extraction of pigments, increased cost of pullulan polysaccharide, high ethanol transportation cost, etc.

Active Publication Date: 2016-03-02
天津慧智百川生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of pullulan polysaccharide consumes a large amount of ethanol, which results in high ethanol transportation costs. In addition, the fermentation of pullulan polysaccharide requires a large amount of industrial water, and the large amount of pigment produced in the late stage of fermentation brings great inconvenience to the subsequent extraction, etc. , these all increase the cost of pullulan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Crushing treatment of corn raw materials, the mass ratio of material to water is 1:2.3, after using double enzymatic saccharification (liquefaction first, then saccharification), adding inorganic salt: (NH 4 ) 2 SO 4 2.0g / L, KH 2 PO 4 1.8g / L, MgSO 4 0.8g / L. Add Saccharomyces cerevisiae and ferment for 55 hours at 30°C. The resulting fermented liquid was distilled at 55°C to obtain ethanol and distiller's grains.

[0024] (2) The measured amount of residual sugar in distiller's grains is about 50 g / L, and the amount of ammonia nitrogen is about 0.5 g / L. Add sucrose 100g / L, 6.0g / LK to it 2 HPO 4 , 0.2g / LMgSO 4 , 2.0 g / L NaCl, adjusted to pH 6.0 (adjusted with 3 mol / L sodium hydroxide and 2 mol / L hydrochloric acid), sterilized at 121° C. for 20 min to prepare a culture medium.

[0025] (3) Preparation of seed medium: 100g / L sucrose, 3.0g / L yeast extract powder, 2.0g / LK 2 HPO 4 , 2.5g / LNaCl, 0.4g / LMgSO 4 , 50.0mg / LFeSO 4 , 1.0g / L (NH 4 ) 2 SO 4 , adjust...

Embodiment 2

[0029] (1) Corn raw materials are crushed and saccharified by double enzyme method, then fermented with Saccharomyces cerevisiae at 32°C for 50 hours. The resulting fermented liquid was distilled at 55°C to obtain ethanol and distiller's grains.

[0030] (2) The measured amount of residual sugar in distiller's grains is about 52g / L, and the amount of ammonia nitrogen is about 0.6g / L. Add sucrose 98g / L, 7.0g / LK to it 2 HPO 4 , 0.4g / LMgSO 4 , 2.0 g / L NaCl, adjusted to pH 6.0 (adjusted with 3 mol / L sodium hydroxide and 2 mol / L hydrochloric acid), sterilized at 121° C. for 20 min to prepare a culture medium.

[0031] (3) Preparation of seed medium: 100g / L sucrose, 3.0g / L yeast extract powder, 2.0g / LK 2 HPO 4 , 2.5g / LNaCl, 0.4g / LMgSO 4 , 50.0mg / LFeSO 4 , 1.0g / L (NH 4 ) 2 SO 4 , adjust the initial pH to 6.0, and sterilize. Insert the activated Aureobasidium pullulans, culture at 28°C, 180r / min for 26h.

[0032] (4) Select the seed solution with the highest OD and put it ...

Embodiment 3

[0035] (1) Corn raw materials are crushed and saccharified by double enzyme method, then fermented with Saccharomyces cerevisiae at 32°C for 50 hours. The resulting fermented liquid was distilled at 55°C to obtain ethanol and distiller's grains.

[0036] (2) The measured amount of residual sugar in distiller's grains is about 55g / L, and the amount of ammonia nitrogen is about 0.7g / L. Add sucrose 95g / L, 7.0g / LK to it 2 HPO 4 , 0.4g / LMgSO 4 , 2.0 g / L NaCl, adjusted to pH 6.0 (adjusted with 3 mol / L sodium hydroxide and 2 mol / L hydrochloric acid), sterilized at 121° C. for 20 min to prepare a culture medium.

[0037] (3) Preparation of seed medium: 100g / L sucrose, 3.0g / L yeast extract powder, 2.0g / LK 2 HPO 4 , 2.5g / LNaCl, 0.4g / LMgSO 4 , 50.0mg / LFeSO 4 , 1.0g / L (NH 4 ) 2 SO 4, adjust the initial pH to 6.0, and sterilize. Insert the activated Aureobasidium pullulans, culture at 28°C, 180r / min for 28h.

[0038] (4) Select the seed solution with the highest OD and put it i...

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PUM

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Abstract

The invention relates to the field of microbial fermentation, and in particular to an ethanol-pullulan coupled two-step fermentation method. The ethanol-pullulan coupled fermentation method mainly comprises the following steps: alcohol fermentation, preparation of culture media by using an alcohol stillage liquid, and pullulan fermentation and extraction. The method comprises the following steps in detail: firstly utilizing a corn raw material to perform alcohol fermentation, stilling the fermentation liquor to obtain ethanol, collecting the alcohol stillage liquid, simply processing the alcohol stillage liquid, using the processed alcohol stillage liquid as the pullulan culture media to ferment, and finally, utilizing the obtained ethanol to perform pullulan extraction. Through the adoption of the ethanol-pullulan coupled fermentation method, the transportation cost of the ethanol can be saved, the remained nutrients in the alcohol stillage liquid is fully recycled, water dosage and generation of pigments in the pullulan fermentation process are reduced, and furthermore, the production cost is reduced.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for coupling two-step fermentation of ethanol-pullulan polysaccharide. Background technique [0002] A large amount of distiller's sap will be produced in the process of fuel ethanol production. At present, most of the processing methods of sap are used for the production of feed or for reflux and reuse, but these methods have the disadvantages of high cost and environmental pollution. In addition, the distiller's grains still contain sugars, abundant free nitrogen and other nutrients, which can supply microorganisms with low nutritional requirements for fermentation. [0003] Pullulan is a microbial exopolysaccharide with important application value. Except for the high concentration of carbon source, the nutritional components required for its fermentation are relatively low. The extraction of pullulan polysaccharide consumes a large amount of ethanol, which resu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P19/10C12R1/645C12R1/865
CPCY02E50/10Y02P20/582
Inventor 乔长晟王萌宋亚琼郝华旋
Owner 天津慧智百川生物工程有限公司